Phosphate → D-Glucosamine

Reaction equation

C([NH:4][C@@H:5]1[C@@H:11]([OH:12])[C@H:10]([OH:13])[C@@H:9]([CH2:14][OH:15])[O:8][CH:6]1[OH:7])(=O)C.P([O-])([O-])([O-])=O>>[OH:7][CH:6]1[O:8][C@H:9]([CH2:14][OH:15])[C@@H:10]([OH:13])[C@H:11]([OH:12])[C@H:5]1[NH2:4]

Participants

Reactant 2

HCID 1061Phosphate

HCID 439174N-Acetyl-D-Glucosamine

Product 1

HCID 439213D-Glucosamine

Procedure

The activity is determined by the following method. Namely, 0.3 ml of a 10% solution of N-acetyl-D-glucosamine is added as a substrate to 0.1 ml of a 200 mM phosphate buffer solution (pH 7.8). Next, 0.1 ml of an enzyme solution is further added thereto followed by incubation at 48° C. for 30 minutes. Then the D-glucosamine thus formed is determined by the indole/hydrochloric acid colorimetry. The amount of the enzyme by which 1 μmol of D-glucosamine is formed in a minute is referred to as 1 U (unit).

Sources & evidence

ORD record: ord-56ee7e03a5e84bf7aaab24c26d7d4d75
Source dataset: uspto-grants-1998_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05731184

Reaction notes

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