Reaction equation
C([NH:4][C@@H:5]1[C@@H:11]([OH:12])[C@H:10]([OH:13])[C@@H:9]([CH2:14][OH:15])[O:8][CH:6]1[OH:7])(=O)C.P([O-])([O-])([O-])=O>>[OH:7][CH:6]1[O:8][C@H:9]([CH2:14][OH:15])[C@@H:10]([OH:13])[C@H:11]([OH:12])[C@H:5]1[NH2:4]Participants
3
Reactant 2
Product 1
Procedure
The activity is determined by the following method. Namely, 0.3 ml of a 10% solution of N-acetyl-D-glucosamine is added as a substrate to 0.1 ml of a 200 mM phosphate buffer solution (pH 7.8). Next, 0.1 ml of an enzyme solution is further added thereto followed by incubation at 48° C. for 30 minutes. Then the D-glucosamine thus formed is determined by the indole/hydrochloric acid colorimetry. The amount of the enzyme by which 1 μmol of D-glucosamine is formed in a minute is referred to as 1 U (unit).
Sources & evidence
- ORD record
- ord-56ee7e03a5e84bf7aaab24c26d7d4d75
- Source dataset
- uspto-grants-1998_03
- Patent number
- US05731184
Linked notes
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