Dimethylformamide → Biotin

HRID 430917

Reaction equation

HRID 430917 reaction equation
C1C(=O)N(OC(CCCCCN[C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@@H:23]2[S:27][CH2:26][C@@H:25]3[NH:28][C:29]([NH:31][C@H:24]23)=[O:30])=[O:18])=O)C(=O)C1.CN(C=[O:36])C>>[OH:36][C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@H:23]1[C@@H:24]2[C@@H:25]([NH:28][C:29]([NH:31]2)=[O:30])[CH2:26][S:27]1)=[O:18]

Participants

3

Reactant 2

HCID 6228DimethylformamideHCID 83874Nhs-LC-biotin

Product 1

HCID 171548Biotin

Procedure

Purified antibodies or their fragments, obtained as described in paragraph A at 1 mg/ml in PBS, were biotinylated for 3 hours at 25° C. (ambient temperature) by adding 37 μl of 10 mg/ml solution of NHS-LC-Biotin, Product #21335; Pierce, Rockford, Ill) in anhydrous DMF. Subsequently, the derivatized antibody was buffer-exchanged on a G25F column into PBS and stored at 0-4° C.

Workup

  1. customPurified antibodies or their fragments
  2. customobtained
  3. customstored at 0-4° C.

Sources & evidence

ORD record
ord-3c314d8a96834c93b9dc8b09d3f343b6
Source dataset
uspto-grants-2003_12
Patent number
US06656744B2

Linked notes

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