Sodium Chloride → Deoxycholic Acid

HRID 481936

Reaction equation

HRID 481936 reaction equation
[CH3:1][C@@H:2]([C@@H:8]1[C@@:12]2([CH3:29])[C@@H:13]([OH:28])[CH2:14][C@@H:15]3[C@@:20]4([CH3:26])[CH2:21][CH2:22][C@@H:23]([OH:25])[CH2:24][C@H:19]4[CH2:18][CH2:17][C@@:16]3(C)[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([O-:7])=[O:6].[Na+].[Na+].[Cl-]>C(O)C(N)(CO)CO.Cl>[CH3:1][C@@H:2]([C@@H:8]1[C@@:12]2([CH3:29])[C@@H:13]([OH:28])[CH2:14][C@@H:15]3[C@@:20]4([CH3:26])[CH2:21][CH2:22][C@@H:23]([OH:25])[CH2:24][C@H:19]4[CH2:18][CH2:17][C@H:16]3[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([OH:7])=[O:6]

Participants

4

Reactant 2

HCID 5234Sodium ChlorideHCID 44582061sodium (4R)-4-[(3R,5R,8R,9R,10S,12S,13R,14S,17R)-3,12-dihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate

Product 1

HCID 222528Deoxycholic Acid

Reagents, catalysts & solvents 1

HCID 93573Tromethamine hydrochlorideSolvent

Procedure

A pellet containing recombinant prothrombin immobilized on Ca3 (PO4)2 was obtained from a prothrombin-containing cell culture, as described above. Five 0.6 gram samples of moist adsorbate were suspended in 3 ml of 20 mM Tris-HCl buffer (pH 8.3) containing 50 mM, 100 mM, 250 mM, 350 mM or 500 mM sodium deoxycholate. Sixty microliter aliquots of 0.76 mg/ml trypsin were added to each sample, and the mixtures were incubated for three hours at room temperature with shaking. After incubation, the buffers of the samples were changed against 0.9% NaCl.

Workup

  1. additionA pellet containing recombinant prothrombin
  2. customwas obtained from a prothrombin-containing cell culture
  3. additionSixty microliter aliquots of 0.76 mg/ml trypsin were added to each sample

Sources & evidence

ORD record
ord-ea5a9a7ae3ab403ea5a1905e0b16be7b
Source dataset
uspto-grants-1998_08
Patent number
US05792623

Linked notes

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