Thiamine Hydrochloride → HCID 178419078, compound

HRID 487047

Reaction equation

HRID 487047 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].[K].S([O-])([O-])(=O)=O.[Mg+2].CC1[N+:32]([CH2:33][C:34]2[CH:35]=[N:36][C:37](C)=[N:38][C:39]=2N)=CSC=1CCO.Cl.[Cl-].[OH:47][C:48](CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:49].C([O-])(=O)C.[NH4+]>S([O-])([O-])(=O)=O.[Mn+2]>[NH2:32][C@H:33]([C:48]([OH:49])=[O:47])[CH2:34][C:35]1[N:36]=[CH:37][NH:38][CH:39]=1

Participants

9

Reactant 7

HCID 6202Thiamine HydrochlorideHCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 517165Ammonium AcetateHCID 5462222PotassiumHCID 6097028Ammonium Sulfate

Product 1

HCID 178419078HCID 178419078, compound

Reagents, catalysts & solvents 1

HCID 24580Manganese SulfateCatalyst

Procedure

An aqueous solution medium having a composition of 10% of glucose, 0.5% of ammonium sulfate, 0.15% of potassium primary phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate, 0.001% of manganese sulfate, 300 μg/l of thiamine hydrochloride, 350 μg/l of biotin, 5 ml/dl of Aji-Eki® and 0.5% of ammonium acetate, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in an autoclave, the various L-histidine-producing bacterial strains shown in Table 14, which had been previously grown in bouillon slants at 30° C. for 24 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 6.5 by the addition of ammonia gas. After completion of the fermentation, the yield of L-histidine produced and accumulated in the solution based on glucose was determined and is shown in Table 14.

Workup

  1. additionat pH 7.0, was charged in an amount of 300 ml
  2. custominto separate small sized glass jar fermenters

Sources & evidence

ORD record
ord-ae9a1e9a8cdf48b497f84afe1eac13bf
Source dataset
uspto-grants-1994_03
Patent number
US05294547

Linked notes

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