o-Dianisidine dihydrochloride → Hexose

HRID 586103

Reaction equation

HRID 586103 reaction equation
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

Participants

4

Reactant 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

Product 1

HCID 206Hexose

Reagents, catalysts & solvents 1

HCID 24243Trisodium PhosphateSolvent2×

Procedure

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidine-dihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

Workup

  1. additionby adding buffer in place of enzyme solution

Sources & evidence

ORD record
ord-654a2f39290e4a00ac02280cb85320bb
Source dataset
uspto-grants-2005_08
Patent number
US06936289B2

Linked notes

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