5'-Adp → 5'-Atp

HRID 67943

Reaction equation

HRID 67943 reaction equation
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:3])[OH:2].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>O>[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([O:7][P:1]([OH:4])([OH:3])=[O:2])([OH:8])=[O:6])(=[O:2])[OH:3]

Participants

4

Reactant 2

HCID 60225'-AdpHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 59575'-Atp

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

The resting cells mentioned above were dried at a room temperature and dried further in a phosphorus pentoxide desiccator under a vacuum overnight. Fifty mg of the dried cells were put into 2 ml of a reaction mixture containing 10 μmoles ADP, 200 μmoles glucose 250 μmoles phosphate buffer, 5 μmoles MgCl2.6H2O and the amount of the inhibitor shown in Table 5 or 6. The reaction mixture was held at 30° C. for 3 hours, and then heated in boiling water for 3 minutes. ATP formed in the reaction mixture was determined colorimetrically.

Workup

  1. customThe resting cells mentioned above were dried at a room temperature
  2. customdried further in a phosphorus pentoxide desiccator under a vacuum overnight
  3. temperatureheated
  4. customfor 3 minutes

Sources & evidence

ORD record
ord-80c7c28f366447698cd962b139a4b19a
Source dataset
uspto-grants-1982_08
Patent number
US04347317

Linked notes

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