2-Chloro-6-methylpyridine-4-carboxylic acid → HCID 178498169, compound
Reaction equation
COC(=O)c1ccc(N)cc1OC.Cc1cc(C(=O)O)cc(Cl)n1.CN1CC[N+](=C1Cl)C.F[P-](F)(F)(F)(F)F.C1=CC2=C(N=C1)N(N=N2)O.CCN(C(C)C)C(C)C.CN(C)C=O>>COC(=O)c1ccc(NC(=O)c2cc(C)nc(Cl)c2)cc1OCParticipants
7
Reactant 2
Product 1
Reagents, catalysts & solvents 4
Conditions
- Temperature
- 25 °C
Workup
- addition150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200.
- addition150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200.
- aliquot10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate.
- addition380 uL MeCN solvent were added to the transfer plate
- aliquot20 uL taken from transfer plate to filter plate 1 for LCMS analysis
- addition180 uL MeCN added to filter plate 1 for LCMS analysis
- aliquot10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis
- addition190 uL MeCN added to filter plate 2 for TOF-DESI analysis
- customthe 96-well filter plates were centrifuged for 2.5 min at 3000 RPM
Sources & evidence
- ORD record
- ord-74ae0bc86c374a90bcaf4579a52cd90b
- Source dataset
- AIChemEco amide coupling conditions 47k dataset
Reaction notes
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