Methane → Adenosine Monophosphate

HRID 897267

Reaction equation

HRID 897267 reaction equation
[CH:1]1[N:2]=[C:3]([NH2:22])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@@H:13]4[CH2:15][O:16][P:17]([OH:20])([O:19][C@H:12]4[C@H:11]3[OH:21])=[O:18])[C:5]=2[N:6]=1.C(O)C(N)(CO)C[OH:26].CN1C(=O)N(C)C(=O)C2NC=NC1=2.CN1C(=O)N(C)C(=O)C2NC=NC1=2.C(N)CN.SCCO.C>>[CH:1]1[N:2]=[C:3]([NH2:22])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([OH:20])([OH:19])=[O:18])[C@@H:12]([OH:26])[C@H:11]3[OH:21])[C:5]=2[N:6]=1

Participants

6

Reactant 5

HCID 297MethaneHCID 15672-MercaptoethanolHCID 6076Camp2×HCID 6503Tris(hydroxymethyl)aminomethaneHCID 9433Aminophylline

Product 1

HCID 6083Adenosine Monophosphate

Procedure

Samples of cAMP standards (0-10 pmol) are added to each test tube containing the incubation buffer (trizma base 0.1 M; aminophylline 8.0 mM; 2-mercaptoethanol 6.0 mM, pH 7.4) and [3H]-cAMP in a total volume of 0.5 mL. The binding protein, previously prepared from beef adrenals, is added to the samples previously incubated at 4° C. for 150 min. and, after the addition of charcoal are centrifuged at 2,000 g for 10 min. The clear supernatant (0.2 mL) is mixed with 4 mL of atomlight in a LS-1800 Beckman scintillation counter.

Workup

  1. customThe binding protein, previously prepared from beef adrenals
  2. additionis added to the samples
  3. waitare centrifuged at 2,000 g for 10 min
  4. additionThe clear supernatant (0.2 mL) is mixed with 4 mL of atomlight in a LS-1800 Beckman scintillation counter

Sources & evidence

ORD record
ord-79ef979748634192b1d906ce1d783b38
Source dataset
uspto-grants-2005_07
Patent number
US06921825B2

Linked notes

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