メタンスルホン酸メチル

EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop.

PRECAUTIONS FOR "CARCINOGENS": Storage site should be as close as practical to lab in which carcinogens are to be used, so that only small quantities required for ... expt need to be carried. Carcinogens should be kept in only one section of cupboard, an explosion-proof refrigerator or freezer (depending on chemicophysical properties ...) that bears appropriate label. An inventory ... should be kept, showing quantity of carcinogen & date it was acquired ... Facilities for dispensing ... should be contiguous to storage area. /Chemical Carcinogens/

PRECAUTIONS FOR "CARCINOGENS": A high-efficiency particulate arrestor (HEPA) or charcoal filters can be used to minimize amt of carcinogen in exhausted air ventilated safety cabinets, lab hoods, glove boxes or animal rooms ... Filter housing that is designed so that used filters can be transferred into plastic bag without contaminating maintenance staff is avail commercially. Filters should be placed in plastic bags immediately after removal ... The plastic bag should be sealed immediately ... The sealed bag should be labelled properly ... Waste liquids ... should be placed or collected in proper containers for disposal. The lid should be secured & the bottles properly labelled. Once filled, bottles should be placed in plastic bag, so that outer surface ... is not contaminated ... The plastic bag should also be sealed & labelled. ... Broken glassware ... should be decontaminated by solvent extraction, by chemical destruction, or in specially designed incinerators. /Chemical Carcinogens/

SMALL SPILLS AND LEAKAGE: If you should spill this chemical, use absorbent paper to pick up all liquid spill material. Seal the absorbent paper, as well as any of your clothing which may be contaminated, in a vapor-tight plastic bag for eventual disposal. Wash any surfaces you may have contaminated with a soap and water solution. Do not reenter the contaminated area until the Safety Officer (or other responsible person) has verified that the area has been properly cleaned.

SRP: The most favorable course of action is to use an alternative chemical product with less inherent propensity for occupational exposure or environmental contamination. Recycle any unused portion of the material for its approved use or return it to the manufacturer or supplier. Ultimate disposal of the chemical must consider: the material's impact on air quality; potential migration in soil or water; effects on animal, aquatic, and plant life; and conformance with environmental and public health regulations.

PRECAUTIONS FOR "CARCINOGENS": There is no universal method of disposal that has been proved satisfactory for all carcinogenic compounds & specific methods of chem destruction ... published have not been tested on all kinds of carcinogen-containing waste. ... summary of avail methods & recommendations ... /given/ must be treated as guide only. /Chemical Carcinogens/

PRECAUTIONS FOR "CARCINOGENS": ... Incineration may be only feasible method for disposal of contaminated laboratory waste from biological expt. However, not all incinerators are suitable for this purpose. The most efficient type ... is probably the gas-fired type, in which a first-stage combustion with a less than stoichiometric air:fuel ratio is followed by a second stage with excess air. Some ... are designed to accept ... aqueous & organic-solvent solutions, otherwise it is necessary ... to absorb soln onto suitable combustible material, such as sawdust. Alternatively, chem destruction may be used, esp when small quantities ... are to be destroyed in laboratory. /Chemical Carcinogens/

PRECAUTIONS FOR "CARCINOGENS": HEPA (high-efficiency particulate arrestor) filters ... can be disposed of by incineration. For spent charcoal filters, the adsorbed material can be stripped off at high temp & carcinogenic wastes generated by this treatment conducted to & burned in an incinerator. ... LIQUID WASTE: ... Disposal should be carried out by incineration at temp that ... ensure complete combustion. SOLID WASTE: Carcasses of lab animals, cage litter & misc solid wastes ... should be disposed of by incineration at temp high enough to ensure destruction of chem carcinogens or their metabolites. /Chemical Carcinogens/

For more Disposal Methods (Complete) data for METHYL METHANESULFONATE (7 total), please visit the HSDB record page.

PRECAUTIONS FOR "CARCINOGENS": Smoking, drinking, eating, storage of food or of food & beverage containers or utensils, & the application of cosmetics should be prohibited in any laboratory. All personnel should remove gloves, if worn, after completion of procedures in which carcinogens have been used. They should ... wash ... hands, preferably using dispensers of liq detergent, & rinse ... thoroughly. Consideration should be given to appropriate methods for cleaning the skin, depending on nature of the contaminant. No standard procedure can be recommended, but the use of organic solvents should be avoided. Safety pipettes should be used for all pipetting. /Chemical Carcinogens/

PRECAUTIONS FOR "CARCINOGENS": In animal laboratory, personnel should remove their outdoor clothes & wear protective suits (preferably disposable, one-piece & close-fitting at ankles & wrists), gloves, hair covering & overshoes. ... Clothing should be changed daily but ... discarded immediately if obvious contamination occurs ... /also,/ workers should shower immediately. In chemical laboratory, gloves & gowns should always be worn ... however, gloves should not be assumed to provide full protection. Carefully fitted masks or respirators may be necessary when working with particulates or gases, & disposable plastic aprons might provide addnl protection. If gowns are of distinctive color, this is a reminder that they should not be worn outside of lab. /Chemical Carcinogens/

PRECAUTIONS FOR "CARCINOGENS": ... Operations connected with synth & purification ... should be carried out under well-ventilated hood. Analytical procedures ... should be carried out with care & vapors evolved during ... procedures should be removed. ... Expert advice should be obtained before existing fume cupboards are used ... & when new fume cupboards are installed. It is desirable that there be means for decreasing the rate of air extraction, so that carcinogenic powders can be handled without ... powder being blown around the hood. Glove boxes should be kept under negative air pressure. Air changes should be adequate, so that concn of vapors of volatile carcinogens will not occur. /Chemical Carcinogens/

PRECAUTIONS FOR "CARCINOGENS": Vertical laminar-flow biological safety cabinets may be used for containment of in vitro procedures ... provided that the exhaust air flow is sufficient to provide an inward air flow at the face opening of the cabinet, & contaminated air plenums that are under positive pressure are leak-tight. Horizontal laminar-flow hoods or safety cabinets, where filtered air is blown across the working area towards the operator, should never be used ... Each cabinet or fume cupboard to be used ... should be tested before work is begun (eg, with fume bomb) & label fixed to it, giving date of test & avg air-flow measured. This test should be repeated periodically & after any structural changes. /Chemical Carcinogens/

For more Preventive Measures (Complete) data for METHYL METHANESULFONATE (9 total), please visit the HSDB record page.

MINIMUM PROTECTIVE CLOTHING: If Tyvek-type disposable protective clothing is not worn during handling of this chemical, wear disposable Tyvek-type sleeves taped to your gloves.

PRECAUTIONS FOR "CARCINOGENS": ... Dispensers of liq detergent /should be available./ ... Safety pipettes should be used for all pipetting. ... In animal laboratory, personnel should ... wear protective suits (preferably disposable, one-piece & close-fitting at ankles & wrists), gloves, hair covering & overshoes. ... In chemical laboratory, gloves & gowns should always be worn ... however, gloves should not be assumed to provide full protection. Carefully fitted masks or respirators may be necessary when working with particulates or gases, & disposable plastic aprons might provide addnl protection. ... Gowns ... /should be/ of distinctive color, this is a reminder that they are not to be worn outside the laboratory. /Chemical Carcinogens/

The combined effects of methyl methanesulfonate and ethyl methanesulfonate on the induction of 6-thioguanine resistant mutants and chromosome aberrations were examined in Chinese hamster V79 cells. Cells were simultaneously treated with ethylmethanesulfonate at a concentration of D20 /SRP: D20 = concentration required to reduce cell survival to 20%/ and methyl methanesulfonate at various concentrations for 3, 6 or 9 hr. In other experiments cells were simultaneously treated with methyl methanesulfonate at a concentration of D20 and ethyl methanesulfonate at various concentrations for 3, 6 or 9 hr. The mathematical analysis of the combined effects of both chemicals for cell killing (cytotoxicity) and 6-thioguanine resistant mutations indicates that synergistic interactions were observed for both cell killing and mutations induced by methyl methanesulfonate and ethyl methanesulfonate. The frequency of chromosome aberrations induced by simultaneous treatment with methyl methanesulfonate at a concentration of D20 and ethyl methanesulfonate at various concentrations for 3 hr was additive. However, the frequency of chromosome aberrations induced by ethyl methanesulfonate at a concentration of D20 and methyl methanesulfonate at various concentrations for 3 hr was not significantly different from those induced by methyl methanesulfonate alone.

Ethanol itself did not induce any apparent chromosome aberrations in Chinese hamster ovary cells. However, post-treatment with ethanol potentiated the chromosome aberrations induced by ... methyl methanesulfonate. ... Chromatid exchanges were predominantly increased in cultures treated with ... methyl methanesulfonate ... and then with ethanol. ... Post-treatment with acetaldehyde, the major metabolite of ethanol, also potentiated the chromosome aberrations induced by ... methyl methanesulfonate. ... The main types of aberrations potentiated by posttreatment with acetaldehyde were similar to those by posttreatment with ethanol. /Methyl methanesulfonate/

EC50; Species: Oryzias latipes (Medaka, high-eyes, embryo 66 hr postfertilization); Conditions: freshwater, static, 20 °C; Concentration: 12.5 mmol/L for 2 hr (95% confidence interval: 10.9-14.0 mmol/L); Effect: growth

LC50; Species: Oryzias latipes (Medaka, high-eyes, embryo 66 hr postfertilization); Conditions: freshwater, static, 20 °C; Concentration: 15.8 mmol/L for 2 hr (95% confidence interval: 14.0-17.8 mmol/L)

LC50 Eisenia fetida (earthworm, mature, weight 370-450 mg) direct application 1-10 ug/sq cm for 48 hr

TERRESTRIAL FATE: Based on a classification scheme(1), an estimated Koc value of 12(SRC), determined from a structure estimation method(2), indicates that methyl methanesulfonate is expected to have very high mobility in soil(SRC). Volatilization of methyl methanesulfonate from moist soil surfaces may occur SRC) given an estimated Henry's Law constant of 4.0X10-6 atm-cu m/mole(SRC), using a fragment constant estimation method(3). However, hydrolysis of methyl methanesulfonate is expected to be the major fate process in moist soil based on measured half-lives in water of 4.56 hours(5), 9.66 hours(6), and 77 hours(7). Methyl methanesulfonate is not expected to volatilize from dry soil surfaces(SRC) based upon an estimated vapor pressure of 0.3 mm Hg(SRC), determined from a fragment constant method(4). Biodegradation data were not available(SRC, 2007).

AQUATIC FATE: Based on a classification scheme(1), an estimated Koc value of 12(SRC), determined from a structure estimation method(2), indicates that methyl methanesulfonate is not expected to adsorb to suspended solids and sediment(SRC). Volatilization from water surfaces is expected(3) based upon an estimated Henry's Law constant of 4.0X10-6 atm-cu m/mole(SRC), developed using a fragment constant estimation method(4). Using this Henry's Law constant and an estimation method(3), volatilization half-lives for a model river and model lake are 6 and 74 days, respectively(SRC). However, the rapid hydrolysis of methyl methanesulfonate, with half-lives of 4.56 hours(9), 9.66 hours(10), and 77 hours(11) in water, indicates that hydrolysis, and not volatilization, will be the major fate process for this compound in aquatic environments. According to a classification scheme(5), an estimated BCF of 3(SRC), from an estimated log Kow of -0.7(6) and a regression-derived equation(7), suggests the potential for bioconcentration in aquatic organisms is low(SRC). Biodegradation data were not available(SRC, 2007).

ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), methyl methanesulfonate, which has an estimated vapor pressure of 0.3 mm Hg at 25 °C(SRC), determined from a fragment constant method(2), is expected to exist solely as a vapor in the ambient atmosphere. Vapor-phase methyl methanesulfonate is degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals(SRC); the half-life for this reaction in air is estimated to be 70 days(SRC), calculated from its rate constant of 2.3X10-13 cu cm/molecule-sec at 25 °C(SRC) that was derived using a structure estimation method(3). Methyl methanesulfonate does not contain chromophores that absorb at wavelengths >290 nm(4) and therefore is not expected to be susceptible to direct photolysis by sunlight(SRC).

Tire leachate water, obtained from tire cores exposed to water for 40 days, did not contain methyl methanesulfonate at concentrations greater than the detection limit of 1.0 ug/L(1). Methyl methanesulfonate was not detected over a two-year period, from 1989-1991, from the incinerator stack gas(both particulate and vapor), the turbine stack gas(both particulate and vapor), offgas from wastewater treatment, or the sweet syngas of a Louisiana gasification plant; GC/MS was used for detection(2).

PRECAUTIONS FOR "CARCINOGENS": Whenever medical surveillance is indicated, in particular when exposure to a carcinogen has occurred, ad hoc decisions should be taken concerning ... /cytogenetic and/or other/ tests that might become useful or mandatory. /Chemical Carcinogens/

Using a structure estimation method based on molecular connectivity indices(1), the Koc of methyl methanesulfonate can be estimated to be 12(SRC). According to a classification scheme(2), this estimated Koc value suggests that methyl methanesulfonate is expected to have very high mobility in soil.

The effects of acute oral exposure to methyl methanesulfonate (MMS) (0, 100, 200, 300 or 400 mg/kg) by gavage in male Wistar rats (20 in control group (water), 5/treated group, number of treated groups not reported) were determined. The rats were necropsied on the 11th day following dosing. There were differences between treated and control groups in the following: reduced body weight gain, increased lethargy and a single death in each group (300 and 400 mg/kg dose-levels). There were differences between treated and control animals at the highest dose level in the following: increase in sperm head count (at 300 mg/kg), and a highly significant increase in the percentage of abnormal sperm heads (all levels) including straight heads (i.e. no hook), excessive curvature, folded, coiled, thin or amorphous heads (and excessively elongated/tapering heads, at high-dose level only). There were no significant differences between treated and control animals in the following: histopathology of the testes or testes weights.

The effects of acute exposure to methyl methanesulfonate (MMS) (0 or 100 mg/kg) by intraperitoneal injection (in water) in male Wistar rats (6/group) were determined. The rats were necropsied on the 11th day following dosing. There were differences between treated and control groups in the following: markedly reduced body weight gain, increased lethargy, reduced testes weights, and a highly significant increase in sperm head count. There was a "qualitative observation" of an increase in sperm head abnormalities.

/HUMAN EXPOSURE STUDIES/ Therapeutic application of total doses of between 2.8-800 mg/kg body weight over a period of up to 350 days to 13 cancer patients led to significant gastrointestinal and hepatic toxic effects.

/EPIDEMIOLOGY STUDIES/ Sixty-one xeroderma pigmentosum patients living in the Federal Republic of Germany were investigated. Clinical symptoms were correlated with DNA repair parameters measured in fibroblasts grown from skin biopsies. Classification according to the international complementation groups revealed that of the 61 patients 3 belonged to group A, 26 to group C, 16 to group D, 3 to group E, and 2 to group F; 11 were of the xeroderma pigmentosum variant type. A striking clinical aspect was the frequency of histogenetically different skin tumors varying from one xeroderma pigmentosum complementation group to the other: squamous and basal cell carinomas predominated in xeroderma pigmentosum group C; lentigo maligna melanomas were most frequent in group D; basal cell carcinomas occurred preferentially in group E and xeroderma pigmentosum variants. Three DNA repair parameters were determined for 46 fibroblast strains: colony forming ability; DNA repair synthesis; and DNA incising capacity. Dose response experiments with up to 13 dose levels were performed throughout to achieve sufficient experimental accuracy. DNA damaging treatments included UV light, the UV like carcinogen N-acetoxy-2-acetylaminofluorene, and the alkylating carcinogens methyl methanesulfonate and N-methyl-N-nitrosourea. Comparison of clinical signs and repair data was made on the basis of colony forming ability, DNA repair synthesis, and DNA incising capacity values of both individual cell strains and weighted means of xeroderma pigmentosum complementation groups. Despite considerable clinical and biochemical heterogeneity within complementation groups distinctive features emerged. In general, colony forming ability, DNA repair synthesis, and DNA incising capacity values of all xeroderma pigmentosum strains investigated, including xeroderma pigmentosum variants, were found to be reduced upon treatment with UV light or N-acetoxy-2-acetylaminofluorene. After treatment with UV light or N-acetoxy-2-acetylaminofluorene, cell strains in which DNA incising capacity was reduced also showed a similar reduction in both colony forming ability and DNA repair synthesis. Consequently, the weighted mean colony forming ability, DNA repair synthesis, and DNA incising capacity values of complementation groups and xeroderma pigmentosum variants correlated with each other. Furthermore, the onset of both early dermatological symptoms of xeroderma pigmentosum and tumor growth correlated with the extent of DNA repair defects. Of 45 xeroderma pigmentosum fibroblast strains checked for colony forming ability after treatment with methyl methanesulfonate only 3 cell strains from group D were found to be more sensitive than normal controls, suggesting that overall repair in xeroderma pigmentosum strains was equal to that in controls. Weighted means of DNA repair synthesis of xeroderma pigmentosum complementation groups, however, showed reductions hinting at impaired excision of distinct alkylated bases. This held true for complementation groups, D, A, E, and F. Upon treatment with N-methyl-N-nitrosourea, the weighted mean DNA repair synthesis values of the complementation groups did not differ significantly from that of controls, ... However, the weighted mean colony forming ability value of complementation group D was significantly reduced, ...

Methyl methanesulfonate

Group 2A: Probably carcinogenic to humans

Volume 7: (1974) Some Anti-thyroid and Related Substances, Nitrofurans and Industrial Chemicals

1999

1998

NB Overall evaluation upgraded to Group 2A with supporting evidence from other relevant data

An estimated BCF of 3 was calculated in fish for methyl methanesulfonate(SRC), using an estimated log Kow of -0.7(1) and a regression-derived equation(2). According to a classification scheme(3), this BCF suggests the potential for bioconcentration in aquatic organisms is low(SRC).

Used as experimental mutagen, teratogen, and brain carcinogen; [HSDB]

Research chemical

Tested clinically as cancer chemotherapeutic agent

Experimentally as mutagen, teratogen and brain carcinogen.

... As catalysts for esterification, polymerizarion, and alkylation, and for the production of peroxycarboxylic acids from hydrogen peroxide and carboxylic acids. /Lower molecular mass alkanesulfonic acids/

(1977) NOT PRODUCED COMMERCIALLY IN US

(1979) NOT PRODUCED COMMERCIALLY IN US

Reaction of mesyl chloride with methanol in anhydrous pyridine.

... Prepared from action of methyl iodide upon methyl sulfite. No indications ... That methyl methanesulfonate is produced commercially ... .