5'-Adp → 5'-Atp

HRID 1249663

反応式

HRID 1249663 の反応式
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:3])[OH:2]>C(OP(O)(O)=O)[C@H]1O[C@H](OP(OP(O)(O)=O)(O)=O)[C@H](O)[C@@H]1O>[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([O:7][P:1]([OH:4])([OH:3])=[O:2])([OH:8])=[O:6])(=[O:2])[OH:3]

参加物

3

反応物 1

HCID 60225'-Adp

生成物 1

HCID 59575'-Atp

試薬・触媒・溶媒 1

HCID 7339Phosphoribosyl Pyrophosphate溶媒3 回

実験手順

The following additions were made to each reaction: 2 μl of PRPP, 2 μl of PRPP Synthetase, and 20 μl PRPP Synthetase buffer. The reactions proceeded at 37° C. for 28 minutes at which time the reactions were transferred to 100 μl LAR containing 2 μl of ADP and 2 μl of NDPK. This second reaction was allowed to proceed at room temperature for 20 min. The amount of ATP produced was measured by the addition of 10 ng of Luciferase followed by measuring light output with a luminometer. The data is presented in Table 30.

後処理

  1. customThe following additions were made to each reaction
  2. customThis second reaction
  3. waitto proceed at room temperature for 20 min

出典と根拠

ORDレコード
ord-e995be76fe084eddae8aedc9e9ed05a6
出典データセット
uspto-grants-2002_01
特許番号
US06335162B1

関連ノート

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