Pyridoxal hydrochloride → Pyridoxal

HRID 1354531

反応式

HRID 1354531 の反応式
CC([O-])=O.[Na+].[CH3:6][C:7]1[C:12]([OH:13])=[C:11]([CH:14]=[O:15])[C:10]([CH2:16][OH:17])=[CH:9][N:8]=1.Cl.[BH3-]C#N.[Na+]>O>[CH3:6][C:7]1[N:8]=[CH:9][C:10]([CH2:16][OH:17])=[C:11]([CH:14]=[O:15])[C:12]=1[OH:13]

参加物

5

反応物 3

HCID 6171Pyridoxal hydrochlorideHCID 517045Sodium AcetateHCID 5003444Sodium cyanoborohydride

生成物 1

HCID 1050Pyridoxal

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

PNA oligomer SEQ ID NO:63 or PNA oligomer SEQ ID NO:64 is treated according to the procedure of Example 62 to convert the N—PHTH group to the free amine. The resulting PNA oligomer is dissolved in 100 microliters of water, and 100 ml of 1M NaOAc buffer (pH 5.0) is added followed by 5 mg of pyridoxal hydrochloride (24 μmols) and 50 μl of 60 mM NaCNBH3 solution. The solution is vortexed, left overnight, and is then passed through a Sephadex G-25 column and further purified in an analytical HPLC column.

後処理

  1. additionPNA oligomer SEQ ID NO:63 or PNA oligomer SEQ ID NO:64 is treated
  2. customfurther purified in an analytical HPLC column

出典と根拠

ORDレコード
ord-9822b87070304004a6400ec5607cd322
出典データセット
uspto-grants-2007_05
特許番号
US07223833B1

関連ノート

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