Acetone → 2-Hydroxyisobutyric acid

HRID 1370158

反応式

HRID 1370158 の反応式
[CH3:1][C:2]([CH3:6])([OH:5])[C:3]#[N:4].C(O)(=[O:10])CC.CC(C)=O.[OH:16][C:17]([CH3:22])([CH3:21])[C:18]([OH:20])=[O:19]>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[OH:16][C:17]([CH3:22])([CH3:21])[C:18]([OH:20])=[O:19].[OH:5][C:2]([CH3:6])([CH3:1])[C:3]([NH2:4])=[O:10]

参加物

7

反応物 4

HCID 180Acetone2 回HCID 1032Propionic AcidHCID 6406Acetone Cyanohydrin3 回HCID 116712-Hydroxyisobutyric acid

生成物 2

HCID 116712-Hydroxyisobutyric acidHCID 83059Propanamide, 2-hydroxy-2-methyl-

試薬・触媒・溶媒 1

HCID 62657Potassium Phosphate, Tribasic溶媒

実験手順

A suspension of 0.383 g (wet cell paste) Comamonas testosteroni 5-MGAM4D cells (ATCC 55744) in 5.56 mL of 50 mM potassium phosphate buffer (pH 6.0) was placed into a 15-mL polypropylene centrifuge tube, then 51.0 mg of acetone cyanohydrin (0.10 M final concentration of acetone cyanohydrin in the suspension) was added and the resulting suspension mixed on a rotating platform at 25° C. Samples for analysis (0.180 mL) were mixed with 0.020 mL of 1.0 M propionic acid (HPLC external standard), centrifuged, and the supernatant analyzed by HPLC for acetone cyanohydrin, acetone, and 2-hydroxyisobutyric acid. After 4 h, the yields of 2-hydroxyisobutyric acid, 2-hydroxyisobutyramide, and acetone were 73.5%, 0%, and 20.4%, respectively, with no acetone cyanohydrin remaining.

後処理

  1. customwas placed into a 15-mL polypropylene centrifuge tube
  2. additionthe resulting suspension mixed on a rotating platform at 25° C

出典と根拠

ORDレコード
ord-07d1ff9aa47a4df7b5e28b67f4c1aef6
出典データセット
uspto-grants-2003_06
特許番号
US06582943B1

関連ノート

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