Ammonia → Coenzyme A

HRID 1453529

反応式

HRID 1453529 の反応式
C([S:18][CH2:19][CH2:20][NH:21][C:22](=[O:65])[CH2:23][CH2:24][NH:25][C:26](=[O:64])[C@H:27]([OH:63])[C:28]([CH3:62])([CH3:61])[CH2:29][O:30][P:31]([OH:60])(=[O:59])[O:32][P:33]([OH:58])(=[O:57])[O:34][CH2:35][C@H:36]1[O:40][C@@H:39]([N:41]2[C:50]3[N:49]=[CH:48][N:47]=[C:45]([NH2:46])[C:44]=3[N:43]=[CH:42]2)[C@H:38]([OH:51])[C@@H:37]1[O:52][P:53]([OH:56])([OH:55])=[O:54])(=O)CCCCCCCCCCCCCCC.N[C@H](C(O)=O)CO.N>C1N(CCO)CCN(CCS(O)(=O)=O)C1.[OH-].[Na+]>[CH3:62][C:28]([C@@H:27]([OH:63])[C:26]([NH:25][CH2:24][CH2:23][C:22]([NH:21][CH2:20][CH2:19][SH:18])=[O:65])=[O:64])([CH2:29][O:30][P:31]([O:32][P:33]([O:34][CH2:35][C@H:36]1[O:40][C@@H:39]([N:41]2[C:50]3[N:49]=[CH:48][N:47]=[C:45]([NH2:46])[C:44]=3[N:43]=[CH:42]2)[C@H:38]([OH:51])[C@@H:37]1[O:52][P:53]([OH:56])([OH:55])=[O:54])([OH:58])=[O:57])([OH:60])=[O:59])[CH3:61]

参加物

5

反応物 3

HCID 222AmmoniaHCID 5951L-SerineHCID 644109palmitoyl CoA

生成物 1

HCID 87642Coenzyme A

試薬・触媒・溶媒 1

HCID 86606154HEPES NaOH溶媒

実験手順

Palmitoyl CoA (10 mg) and 1 mg of L-serine were dissolved in 5 mL of 100 mM HEPES-NaOH buffer (containing 10 μM PLP; pH 8.0) and well stirred using a magnetic stirrer. While the stirring speed was reduced to such an extent that a mild mixing was resulted, 0.5 mL of a crude SPT enzyme solution was added and the mixture was made to react at 37° C. for 24 hours. The solution was made alkaline with 1 mL of 2N ammonia solution and the product in the solution was extracted and recovered with 5 mL of chloroform/methanol (2:1, (v/v).). The extract liquid was filtered, concentrated appropriately and analyzed by the same manner as in Example 3 whereupon the produced amount of 3-ketodihydrosphingosine was about 0.02 mg.

後処理

  1. additiona mild mixing
  2. customwas resulted
  3. addition0.5 mL of a crude SPT enzyme solution was added
  4. customto react at 37° C. for 24 hours
  5. extractionthe product in the solution was extracted
  6. customrecovered with 5 mL of chloroform/methanol (2:1, (v/v).)
  7. filtrationThe extract liquid was filtered
  8. concentrationconcentrated appropriately

出典と根拠

ORDレコード
ord-297cdc40d817453f8e7517e972ef4d85
出典データセット
uspto-grants-2009_01
特許番号
US07476521B2

関連ノート

読み込み中…