Potassium Hydroxide → Morphine

HRID 1458505

反応式

HRID 1458505 の反応式
Cl.[CH:2]1[C:18]2[CH2:17][C@H:16]3[N:19]([CH2:21][CH2:22][C@@:8]45[C@H:15]3[CH:14]=[CH:13][C@H:11]([OH:12])[C@@H:9]4[O:10][C:6]([C:7]=25)=[C:4]([OH:5])[CH:3]=1)[CH3:20].[OH-].[K+].C1C(CN2C(=O)C=CC2=O)CCC(C(ON2C(=O)CCC2=O)=O)C1>CN(C)C=O>[CH:2]1[C:18]2[CH2:17][C@H:16]3[N:19]([CH2:21][CH2:22][C@@:8]45[C@H:15]3[CH:14]=[CH:13][C@H:11]([OH:12])[C@@H:9]4[O:10][C:6]([C:7]=25)=[C:4]([OH:5])[CH:3]=1)[CH3:20]

参加物

5

反応物 3

HCID 14797Potassium HydroxideHCID 125175Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylateHCID 5464110Morphine Hydrochloride

生成物 1

HCID 5288826Morphine

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒

実験手順

Morphine-HCTL (0.068 g, 1.3×10-4 mol, example 9) was dissolved in 5.7 ml of 70% dimethylformamide/30% water and 1.43 ml 1N potassium hydroxide was added. After 5 min the thiol concentration was determined by reaction with DTNB to be 16.9 mM. The morphine thiol -- (6.4 ml, 1.1×10-4 mol) was added with stirring to 26.7 ml SMCC-BSA, 15/1, (0.16 g, 2.4×10-6 mol). The container was purged with argon and the solution was stirred at room temperature for 2 hr. The protein solution was then dialyzed using a PYROSART module against 10 mM (2-(N-morpholino) ethane sulfonic acid, pH 5.77, and 29.5 ml of 5.39 mg/ml BSA-morphine was collected. The protein solution was frozen at -70° C.

後処理

  1. concentrationAfter 5 min the thiol concentration
  2. customwas determined by reaction with DTNB
  3. customThe container was purged with argon
  4. customThe protein solution was then dialyzed
  5. customwas collected
  6. temperatureThe protein solution was frozen at -70° C.

出典と根拠

ORDレコード
ord-a35981d8995646fbb08b45f0161ebb34
出典データセット
uspto-grants-1998_12
特許番号
US05851776

関連ノート

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