Sodium Chloride → 2'-Deoxycytidine-5'-Monophosphate

HRID 1703536

反応式

HRID 1703536 の反応式
C1[C@H](N2C3NC=NC(=O)C=3N=C2)O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@H]1O.[P:31]([O:43][CH2:44][C@H:45]1[O:49][C@@H:48]([N:50]2[CH:57]=[CH:56][C:54]([NH2:55])=[N:53][C:51]2=[O:52])[CH2:47][C@@H:46]1[OH:58])([O:34]P(OP(O)(O)=O)(O)=O)(=[O:33])[OH:32].P([O-])([O-])([O-])=O.[K+].[K+].[K+].[Cl-].[Mg+2].[Cl-].[Cl-].[Na+].C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.[Na+].[Na+].[Na+]>>[CH2:47]1[C@H:48]([N:50]2[C:51](=[O:52])[N:53]=[C:54]([NH2:55])[CH:56]=[CH:57]2)[O:49][C@H:45]([CH2:44][O:43][P:31]([OH:33])([OH:34])=[O:32])[C@H:46]1[OH:58]

参加物

7

反応物 6

HCID 5234Sodium ChlorideHCID 6224Trisodium citrateHCID 62657Potassium Phosphate, TribasicHCID 65091dCTPHCID 5360315Magnesium ChlorideHCID 178518211HCID 178518211、化合物

生成物 1

HCID 139452'-Deoxycytidine-5'-Monophosphate

反応条件

温度
-20 °C

実験手順

A reaction mixture for the synthesis of poly (dI:dC) contains 3.5 μ moles each of dITP and deoxycytidine-5'-triphosphate (dCTP), 600 μ moles of potassium phosphate buffer (pH 7.4) and 30 μ moles of magnesium chloride, and 40-80 units of DNA (deoxynucleic acid) polymerase in a total volume of 10 ml. After a lag period of 2-4 hours, polymerization is complete in 6-8 hours. The course of the reaction is followed by optical density measurements at intervals. When hypochromicity at 260 mμ is at a maximum, the reaction is stopped by adjusting the mixture to 0.2M sodium chloride and 0.1M sodium citrate. Dialysis is extensively carried out against the same buffer to remove all low molecular weight material. The product is stored frozen at -20° C. until used for interferon induction.

後処理

  1. customA reaction mixture for the synthesis of poly (dI:dC)
  2. customAfter a lag period of 2-4 hours, polymerization
  3. customDialysis
  4. customto remove all low molecular weight material

出典と根拠

ORDレコード
ord-13ca69f532d84eb8b6efb15825b8ecb0
出典データセット
uspto-grants-1978_11
特許番号
US04124702

関連ノート

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