5'-Atp → 5'-Adp
反応式
[Mg+2].[Cl-].[Cl-].C(O)C(N)(CO)CO.Cl.[P:13]([O:25][CH2:26][C@H:27]1[O:31][C@@H:30]([N:32]2[C:41]3[N:40]=[CH:39][N:38]=[C:36]([NH2:37])[C:35]=3[N:34]=[CH:33]2)[C@H:29]([OH:42])[C@@H:28]1[OH:43])([O:16][P:17]([O:20]P(O)(O)=O)([OH:19])=[O:18])(=[O:15])[OH:14]>>[P:13]([O:25][CH2:26][C@H:27]1[O:31][C@@H:30]([N:32]2[C:41]3[N:40]=[CH:39][N:38]=[C:36]([NH2:37])[C:35]=3[N:34]=[CH:33]2)[C@H:29]([OH:42])[C@@H:28]1[OH:43])([O:16][P:17]([OH:19])([OH:20])=[O:18])(=[O:14])[OH:15]参加物
4
実験手順
Next, 50 μl of a reaction mixture containing 0.16 μg of the apyrase-treated PPK-ADK, 10 μM AMP, 400 μM polyphosphate, 8 mM MgCl2, and 60 mM Tris-HCl (pH 7.4) were prepared. Then, 5 μl of the reaction mixture were sampled and mixed with 40 μl of an ATP bioluminescence assay reagent (Roche), and luminescence was measured immediately by using a multiplate luminometer (ARVO, Wallac).
後処理
- customwere prepared