A solution of Example 224b (76.1 mg, 0.122 mmol) in dioxane (1.5 mL) was treated with 20% sodium hydroxide (0.10 mL, 0.122 mmol). The mixture was heated at 90° C. for 1 hour. The solvent was evaporated. The residue was treated with 20% brine and extracted with EtOAc (2×). The combined organic layers were dried. The crude intermediate was dissolved in CH2Cl2 (1.0 mL) and treated with TFA (0.19 mL, 2.4 mmol). After 1 hour, the reaction mixture was concentrated and purified by reverse-phase HPLC as described in Example 56 to give the title compound (6.5 mg) as trifluoroacetic acid salts. 1H NMR (400 MHz, CD3OD) ppm 1.29-1.43 (m, 2H), 1.43-1.49 (m, 3H), 1.52-1.72 (m, 2H), 2.02-2.17 (m, 2H), 2.17-2.32 (m, 2H), 3.13 (m, 1H), 3.80 (m, 1H), 4.18 (q, J=7.02 Hz, 2H), 6.75 (d, J=1.22 Hz, 1H), 6.86 (d, J=1.22 Hz, 1H), 7.86 (s, 1H), 7.90 (d, J=2.44 Hz, 1H), 8.06 (d, J=2.44 Hz, 1H). MS (DCI+) m/z 386.2 (M+H)+.
