反応式
C(S)[C@@H](O)[C@H](O)CS.[C:9]([O-:14])(=[O:13])[C:10]([CH3:12])=[O:11].[O:15]=[CH:16][C@H:17]([C@H:19]([C@@H:21]([C@@H:23]([CH2:25][OH:26])[OH:24])[OH:22])[OH:20])[OH:18].OC(C1(O[C@@H]([C@@H]([C@@H](CO)O)O)[C@H](NC(C)=O)[C@@H](O)C1)O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[C:9]([OH:14])(=[O:13])[C:10]([CH2:12][C@H:25]([C@@H:23]([C@@H:21]([C@H:19]([C@H:17]([CH2:16][OH:15])[OH:18])[OH:20])[OH:22])[OH:24])[OH:26])=[O:11]参加物
6
反応条件
- 温度
- 37 °C
実験手順
To a 10 mL potassium phosphate buffer (0.1M, pH 7.5) were added 10 mM DTT, 0.5M pyruvate, 0.1M D-mannose and 1.5 mg NeuAc aldolase. The reaction mixture was shaken at 37° C. for three days. The reaction mixture was chromatographed with a Dowex-1 (HCO3-) resin, eluted with zero to 1M ammonium bicarbonate gradient. The fractions containing KDN were pooled and lyophilized three times repeatedly to remove the volatile salt to give a 78 percent yield of KDN. The physical data of the product were are consistent with the reported.
後処理
- customThe reaction mixture was chromatographed with a Dowex-1 (HCO3-) resin
- washeluted with zero to 1M ammonium bicarbonate gradient
- additionThe fractions containing KDN
- customto remove the volatile salt
- customto give a 78 percent yield of KDN
出典と根拠
- ORDレコード
- ord-cb099647c5f04bdc86a3205ac0524140
- 出典データセット
- uspto-grants-1995_10
- 特許番号
- US05461143
関連ノート
読み込み中…