NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 223250

反応式

HRID 223250 の反応式
C(O)C(N)(CO)CO.Cl.[CH:10]1[CH:15]=[N+:14]([C@@H:16]2[O:20][C@H:19]([CH2:21][O:22][P:23]([O:26][P:27]([O:30][CH2:31][C@H:32]3[O:36][C@@H:35]([N:37]4[C:41]5[N:42]=[CH:43][N:44]=[C:45]([NH2:46])[C:40]=5[N:39]=[CH:38]4)[C@H:34]([O:47][P:48]([OH:51])([OH:50])=[O:49])[C@@H:33]3[OH:52])([OH:29])=[O:28])([OH:25])=[O:24])[C@@H:18]([OH:53])[C@H:17]2[OH:54])[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH:11]=1>C(O)(C)C>[CH:43]1[N:44]=[C:45]([NH2:46])[C:40]2[N:39]=[CH:38][N:37]([C@@H:35]3[O:36][C@H:32]([CH2:31][O:30][P:27]([O:26][P:23]([O:22][CH2:21][C@H:19]4[O:20][C@@H:16]([N:14]5[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH2:11][CH:10]=[CH:15]5)[C@H:17]([OH:54])[C@@H:18]4[OH:53])([OH:25])=[O:24])([OH:29])=[O:28])[C@@H:33]([OH:52])[C@H:34]3[O:47][P:48]([OH:51])([OH:50])=[O:49])[C:41]=2[N:42]=1

参加物

4

反応物 2

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 93573Tromethamine hydrochloride

生成物 1

HCID 5884NADPH

試薬・触媒・溶媒 1

HCID 3776Isopropanol溶媒

反応条件

温度
45 °C

実験手順

The gel was used to prepare a sepharose-alcohol dehydrogenase column (0.4×4.0 cm) which was then immersed in a water bath maintained at 45° C. A 0.01 M Tris-HCl solution (pH 9.0) containing 2.5 mg/ml NADP and 0.25 v/v isopropanol was applied to the column at a rate of 0.25 ml/min.; the treated solution was collected in a cooled ethanol precipitation vessel. Several milliliters of petroleum ether were added to 20 ml of the eluted solution and the precipitate was collected by centrifugation.

後処理

  1. customwas then immersed in a water bath
  2. customthe treated solution was collected in a cooled ethanol precipitation vessel
  3. additionSeveral milliliters of petroleum ether were added to 20 ml of the eluted solution
  4. customthe precipitate was collected by centrifugation

出典と根拠

ORDレコード
ord-509c63b7c80e462a862f4e786f5ff757
出典データセット
uspto-grants-1982_10
特許番号
US04352885

関連ノート

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