Tris(hydroxymethyl)aminomethane → D-(-)-Fructose

HRID 223585

反応式

HRID 223585 の反応式
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].C(O)C(N)(CO)CO.C(O)(=O)/C=C\C(O)=O.OS([O-])(=O)=O.[Na+]>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

参加物

5

反応物 4

HCID 6503Tris(hydroxymethyl)aminomethaneHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2 回HCID 444266Maleic AcidHCID 516919Sodium Bisulfate

生成物 1

HCID 5984D-(-)-Fructose

実験手順

The glucose isomerase activity of the immobilized samples was estimated at 70° C. using a batch assay technique using small particles (≈80 mesh). The assay was carried out in 125 ml. Erlenmeyer flasks in which the dry enzyme was hydrated in substrate (1 M glucose, 0.1 M Tris, 0.1 M maleic acid, 4.1 mM MgSO4.7H2O and 5 mmM NaHSO4 at pH 8.0) for 10 minutes at room temperature. The assay reaction was started by placing the flask in a 70° C. shaker water bath. At 15, 30, 45, 60 and 75 minutes, a 0.05 ml. sample of the reaction mixture was removed and added to 1.0 ml. of 0.1M HClO4 solution to stop the reaction. The amount of fructose formed in each sample was then estimated by the cysteine H2SO4 method. The activity was calculated through regression analysis of fructose formed with respect to time, where a unit of activity represents one μ mole of fructose formed per minute under conditions of the assay.

後処理

  1. customwas estimated at 70° C.
  2. customThe assay reaction
  3. customin a 70° C.
  4. customAt 15, 30, 45, 60 and 75 minutes
  5. customsample of the reaction mixture was removed
  6. additionadded to 1.0 ml
  7. customthe reaction

出典と根拠

ORDレコード
ord-694cc5f170234a7ebc4259aab84863ab
出典データセット
uspto-grants-1982_10
特許番号
US04355105

関連ノート

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