Carbon Dioxide → [(4S,6R,9E,11R,12R)-11-[(2R,3R,4R,5R,6R)-4,5-dihydroxy-6-methyl-3-(methylamino)oxan-2-yl]oxy-4-[(4R)-2-oxo-1,3-dioxolan-4-yl]-5-oxatricyclo[8.3.0.04,6]trideca-1(13),9-dien-2,7-diyn-12-yl] 7-methoxy-5-methylnaphthalene-1-carboxylate

HRID 2237396

反応式

HRID 2237396 の反応式
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)[OH:8])[OH:6])[OH:4].[C:13]([O-])(=O)[C:14]([CH3:16])=O.CC1(C)S[C@@H]2[C@H](N[C:29]([CH2:31][C:32]3[CH:33]=[CH:34][CH:35]=[CH:36][CH:37]=3)=[O:30])C(=O)N2[C@H]1C([O-])=O.[K+].C[C@@H]1O[C@@H:47]([O:49][C@H:50]2[C@H:55](O)[C@@H:54](O)[C@H:53](NC(N)=N)[C@@H:52](O)[C@@H:51]2NC(N)=N)[C@H:46]([O:67][C@@H:68]2[O:73][C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@:45]1([OH:82])C=O.C[C@H](N)[C:85]([NH:87][C@H](C(O)=O)CCC(N)=O)=O.[C:98](=O)=O.CC[C:103]([O:105][C:106]1([C:113]2[CH:118]=[CH:117][CH:116]=CC=2)CCN(C)CC1)=[O:104]>>[CH3:13][C:14]1[C:37]2=[CH:36][CH:35]=[CH:34][C:33]([C:103]([O:105][C@H:106]3[C@H:51]([O:12][C@H:11]4[O:4][C@H:3]([CH3:2])[C@H:5]([OH:6])[C@H:7]([OH:8])[C@H:9]4[NH:87][CH3:85])[C:52]4=[CH:53][C:54]#[C:55][C@H:50]5[O:49][C@@:47]5([C@@H:46]5[O:67][C:68](=[O:73])[O:82][CH2:45]5)[C:116]#[C:117][C:118]4=[CH:113]3)=[O:104])=[C:32]2[CH:31]=[C:29]([O:30][CH3:98])[CH:16]=1

参加物

8

反応物 7

HCID 280Carbon DioxideHCID 615834-propionyloxy-4-phenyl-N-methylpiperidineHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 107735PyruvateHCID 123935L-Alanyl-L-GlutamineHCID 23664709Penicillin G PotassiumHCID 178484872HCID 178484872、化合物

生成物 1

HCID 44581473[(4S,6R,9E,11R,12R)-11-[(2R,3R,4R,5R,6R)-4,5-dihydroxy-6-methyl-3-(methylamino)oxan-2-yl]oxy-4-[(4R)-2-oxo-1,3-dioxolan-4-yl]-5-oxatricyclo[8.3.0.04,6]trideca-1(13),9-dien-2,7-diyn-12-yl] 7-methoxy-5-methylnaphthalene-1-carboxylate

実験手順

Human cervix adenocarcinoma HeLa cells are maintained as exponentially growing monolayers in Dulbecco modified Eagle's minimum essential medium with 4.5 g/l glucose, 0.1 g/l pyruvate, 105 UI/I penicilline, 0.1 g/l streptomycine, 0.86 g/l Glutamax I and 10% v/v foetal calf serum (37° C., 5% CO2), without or with 5 μM DL-threo-1-phenyl-2-hexadecanoyl-amino-3-morpholino-1-propanol (PPMP) to inhibit Gb3 expression. When present, PPMP is introduced into pre-cultures for at least 6 days prior to cytotoxicity assays and is present for up to drug wash.

後処理

  1. temperatureHuman cervix adenocarcinoma HeLa cells are maintained as exponentially growing monolayers in Dulbecco
  2. washwash

出典と根拠

ORDレコード
ord-eea5a276962d4863a6890568a670443e
出典データセット
uspto-grants-2010_05
特許番号
US07718601B2

関連ノート

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