Hydrochloric Acid → Thromboxane A2

HRID 2303938

反応式

HRID 2303938 の反応式
C(O)C(N)(CO)CO.Cl.C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.[CH3:30][CH2:31][CH2:32][CH2:33][CH2:34][C@H:35]([OH:54])/[CH:36]=[CH:37]/[C@@H:38]1[C@@H:42]([CH2:43]/[CH:44]=[CH:45]\[CH2:46][CH2:47][CH2:48][C:49]([OH:51])=[O:50])[C@H:41]2[O:52][O:53][C@@H:39]1[CH2:40]2.Cl.C(O)C(N)(CO)CO.CCCCC[C@H](O)/C=C/[C@H]1OC(O)C[C@H](O)[C@@H]1C/C=C\CCCC(O)=O>>[CH3:30][CH2:31][CH2:32][CH2:33][CH2:34][C@H:35]([OH:54])/[CH:36]=[CH:37]/[C@H:38]1[O:53][C@@H:39]2[O:52][C@@H:41]([CH2:40]2)[C@@H:42]1[CH2:43]/[CH:44]=[CH:45]\[CH2:46][CH2:47][CH2:48][C:49]([OH:51])=[O:50]

参加物

7

反応物 6

HCID 313Hydrochloric AcidHCID 6049Ethylenediaminetetraacetic AcidHCID 6503Tris(hydroxymethyl)aminomethaneHCID 93573Tromethamine hydrochlorideHCID 445049Prostaglandin H2HCID 5283137Thromboxane B2

生成物 1

HCID 5280497Thromboxane A2

実験手順

1 ml of a buffer (20 MM Tris-HCl buffer, 1 mM EDTA, pH 7.5) containing human blood platelet microsomes (50 μg protein/ml) and the test compound (final concentration 10−7M) was agitated, then incubated at 0° C. for 30 minutes. To this was added prostaglandin H2 (100 ng/2 μl). This was incubated at 23° C. for 3 minutes to cause a reaction. Next, 1M hydrochloric acid was added to make the solution acidic and stop the reaction, then this was neutralized by 1M Tris-Base and centrifuged at 3000 rpm for 20 minutes. The amount of the TxB2 in the supernatant was measured by the EIA method (Cayman Co. kit).

後処理

  1. waitThis was incubated at 23° C. for 3 minutes
  2. customa reaction
  3. customthe reaction
  4. waitcentrifuged at 3000 rpm for 20 minutes

出典と根拠

ORDレコード
ord-29c71b802d754eff95957135ab6dd020
出典データセット
uspto-grants-2002_08
特許番号
US06433167B1

関連ノート

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