Phosphate → L-(-)-Ornithine

HRID 232509

反応式

HRID 232509 の反応式
[NH2:1][CH:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6]C(=N)N.O=C1O[C@H]([C@H](CO)O)C(O)=C1O.P([O-])([O-])([O-])=O.N[C@H](C(O)=O)CCCNC(=N)N>O.S([O-])([O-])(=O)=O.[Mn+2].C(#N)C>[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH2:6]

参加物

8

反応物 4

HCID 1061PhosphateHCID 54670067L-Ascorbic AcidHCID 178418600HCID 178418600、化合物HCID 178512957HCID 178512957、化合物

生成物 1

HCID 6262L-(-)-Ornithine

試薬・触媒・溶媒 3

HCID 24580Manganese Sulfate触媒HCID 962Water溶媒2 回HCID 6342Acetonitrile溶媒

実験手順

1.74 g DL-arginine (50:50) were dissolved in 10 ml water and mixed with 0.21 mg manganese sulfate.H2O, 0.11 mg ascorbic acid and 0.5 mg calf liver L-arginase (Boeringer Mannheim, 97 U/mg. The progress of the reaction was followed with HPLC (amino column; mobile solvent 65:35 acetonitrile:phosphate buffer (pH 6.4). After 6 hours at 25° C., 50% of the original amount of arginine had reacted and a corresponding amount of ornithine had been formed (conversion 100%).

出典と根拠

ORDレコード
ord-d0f7a5dd9a9e4a499ca06ed3a43f1697
出典データセット
uspto-grants-1997_01
特許番号
US05591613

関連ノート

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