Ethyl chloroformate → HCID 86594860、化合物
反応式
Cl[C:2]([O:4][CH2:5][CH3:6])=[O:3].[CH:7]1([O:12][C:13]2[CH:18]=[CH:17][C:16]([NH:19][C:20]([NH:22][C:23]3[CH:28]=[CH:27][C:26]([N:29]4[CH2:33][CH2:32][CH:31](NC)[CH2:30]4)=[CH:25][CH:24]=3)=[O:21])=[CH:15][CH:14]=2)[CH2:11][CH2:10][CH2:9][CH2:8]1.C[CH2:37][N:38](C(C)C)C(C)C>ClCCl>[CH:7]1([O:12][C:13]2[CH:14]=[CH:15][C:16]([NH:19][C:20](=[O:21])[NH:22][C:23]3[CH:28]=[CH:27][C:26]([N:29]4[CH2:33][CH2:32][CH:31]([CH2:37][NH:38][C:2](=[O:3])[O:4][CH2:5][CH3:6])[CH2:30]4)=[CH:25][CH:24]=3)=[CH:17][CH:18]=2)[CH2:8][CH2:9][CH2:10][CH2:11]1参加物
5
実験手順
Ethyl chloroformate (8 μl) was added dropwise to a solution of 1-(4-cyclopentyloxyphenyl )-3-[4-(3-methylaminopyrrolidin-1-yl)phenyl]urea (20 mg) and Hünig's base (10 mg) in dichloromethane (3 ml). After 12 hours, the reaction mixture was concentrated and the residue was purified by preparative HPLC. This resulted in the product with the molecular weight of 466.59 (C26H34N4O4); MS (ESI): 467 (M+H+) as hydrotrifluoroacetate.