Trifluoroacetic acid → Lys-Val-Lys

HRID 251316

反応式

HRID 251316 の反応式
CC(OC([NH:8][CH2:9][CH2:10][CH2:11][CH2:12][C@H:13]([NH2:17])[C:14]([OH:16])=[O:15])=O)(C)C.[NH:18]([C:26]([O:28]CC1C2C(=CC=CC=2)C2C1=CC=CC=2)=O)[C@H:19]([C:23]([OH:25])=O)[CH:20]([CH3:22])[CH3:21].[NH:43](C(OCC1C2C(=CC=CC=2)C2C1=CC=CC=2)=O)[C@H:44](C(O)=O)[CH2:45][CH2:46][CH2:47][CH2:48][NH:49]C(OC(C)(C)C)=O.C(O)(C(F)(F)F)=O>CCOCC>[NH2:43][C@H:44]([C:26]([NH:18][C@H:19]([C:23]([NH:17][C@H:13]([C:14]([OH:16])=[O:15])[CH2:12][CH2:11][CH2:10][CH2:9][NH2:8])=[O:25])[CH:20]([CH3:21])[CH3:22])=[O:28])[CH2:45][CH2:46][CH2:47][CH2:48][NH2:49]

参加物

6

反応物 4

HCID 6422Trifluoroacetic acidHCID 688217FMOC-L-valineHCID 2724628Fmoc-Lys(Boc)-OHHCID 2733283(2S)-2-amino-6-(((tert-butoxy)carbonyl)amino)hexanoic acid

生成物 1

HCID 448140Lys-Val-Lys

試薬・触媒・溶媒 1

HCID 3283Diethyl Ether溶媒

実験手順

The protective peptide is built on 1.00 g (0.80 mmol) of H-Lys(Boc)-2-chlorotrityl resin using Fmoc-Val-OH, Fmoc-Lys(Boc)-OH and Palm-OSu. The resin is treated for 30 min with 8 ml of TFA 95% and the solution is instilled in 100 ml of Et2O. The precipitate is sucked off, washed and purified by prep. HPLC after drying. The substance is diluted in 30 ml of dioxan:water=4:6, treated overnight with 2.0 g of BioRad resin (acetate form), filtered, rotated and lyophilized. Yield: 110 mg (0.15 mmol, 19%)

後処理

  1. washwashed
  2. custompurified by prep
  3. customHPLC after drying
  4. additionThe substance is diluted in 30 ml of dioxan
  5. additionwater=4:6, treated overnight with 2.0 g of BioRad resin (acetate form)
  6. filtrationfiltered

出典と根拠

ORDレコード
ord-73c73a56b2d444b7857252e72f7b484d
出典データセット
uspto-grants-2011_01
特許番号
US07863417B2

関連ノート

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