Uridine Diphosphate → GlyTouCan:G04544UD

HRID 257720

反応式

HRID 257720 の反応式
P([O-])([O-])(O[CH:4]1[O:11][C@H:10]([CH2:12][OH:13])[C@@H:8]([OH:9])[C@H:6]([OH:7])[CH2:5]1)=O.[C:16]([NH:19][C@@H:20]1[C@@H:26]([OH:27])[C@H:25]([OH:28])[C@@H:24]([CH2:29][OH:30])[O:23][CH:21]1[OH:22])(=[O:18])[CH3:17].C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.C(OP(O)(O)=O)[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O>>[C:16]([NH:19][C@@H:20]1[C@@H:26]([OH:27])[C@H:25]([O:28][C@@H:4]2[O:11][C@H:10]([CH2:12][OH:13])[C@H:8]([OH:9])[C@H:6]([OH:7])[CH2:5]2)[C@@H:24]([CH2:29][OH:30])[O:23][CH:21]1[OH:22])(=[O:18])[CH3:17]

参加物

5

反応物 4

HCID 6031Uridine DiphosphateHCID 82400Glucose 1,6-bisphosphateHCID 439174N-Acetyl-D-GlucosamineHCID 867473842-Deoxy-D-glucopyranosyl 1-phosphate

生成物 1

HCID 15929800GlyTouCan:G04544UD

実験手順

2-Deoxyglucose 6-phosphate (2, 60 mg, 190 μmol), N-acetylglucosamine (6, 71 mg, 321 μmol), UDP (2 mg, 5 μmol), PEP (44 mg, 210 μmol), glucose 1, 6-diphosphate (0.1 mg) and bovine serum albumin (BSA, 5 mg) are dissolved in 10 ml of deoxygenated buffer. The enzymes are subsequently added:

後処理

  1. additionThe enzymes are subsequently added

出典と根拠

ORDレコード
ord-871cd89d6b384189a2a1c5469cb81c82
出典データセット
uspto-grants-1993_11
特許番号
US05264352

関連ノート

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