Water → H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

HRID 358157

反応式

HRID 358157 の反応式
OP([O-])([O-])=O.[K+].[K+].OP([O-])(O)=O.[K+].ClC1N=C(Cl)N=C(Cl)N=1.C[CH2:24][C@@H:25]([C@H:27]([NH:77][C:78]([C@@H:80]([NH:89][C:90]([C@@H:92]([NH:96][C:97]([C@@H:99]([NH:107][C:108]([C@@H:110]([NH2:115])[CH2:111][C:112]([OH:114])=[O:113])=[O:109])[CH2:100][CH2:101][CH2:102][N:103]=[C:104]([NH2:106])[NH2:105])=[O:98])[CH:93]([CH3:95])[CH3:94])=[O:91])[CH2:81][C:82]1[CH:87]=[CH:86][C:85]([OH:88])=[CH:84][CH:83]=1)=[O:79])[C:28]([NH:30][C@H:31]([C:38]([N:40]1[C@H:44]([C:45]([NH:47][C@H:48]([C:56](N[C@H](C(N[C@H](C(O)=O)CC(C)C)=O)CC2NC=NC=2)=[O:57])[CH2:49]C2C=CC=CC=2)=[O:46])[CH2:43][CH2:42][CH2:41]1)=[O:39])[CH2:32][C:33]1[NH:37][CH:36]=[N:35][CH:34]=1)=[O:29])[CH3:26].[OH2:116]>O1CCOCC1.CO>[CH3:49][C@H:48]([NH:47][C:45]([C@H:44]1[N:40]([C:38]([C@@H:31]([NH:30][C:28]([C@@H:27]([NH:77][C:78]([C@@H:80]([NH:89][C:90]([C@@H:92]([NH:96][C:97]([C@@H:99]([NH:107][C:108]([C@@H:110]([NH2:115])[CH2:111][C:112]([OH:114])=[O:113])=[O:109])[CH2:100][CH2:101][CH2:102][NH:103][C:104]([NH2:106])=[NH:105])=[O:98])[CH:93]([CH3:94])[CH3:95])=[O:91])[CH2:81][C:82]2[CH:87]=[CH:86][C:85]([OH:88])=[CH:84][CH:83]=2)=[O:79])[CH:25]([CH3:24])[CH3:26])=[O:29])[CH2:32][C:33]2[NH:37][CH:36]=[N:35][CH:34]=2)=[O:39])[CH2:41][CH2:42][CH2:43]1)=[O:46])[C:56]([OH:57])=[O:116]

参加物

8

反応物 5

HCID 962Water2 回HCID 7954Cyanuric chlorideHCID 24450Potassium Phosphate, DibasicHCID 516951Monobasic potassium phosphateHCID 3081372Angiotensin I

生成物 1

HCID 178448346H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

試薬・触媒・溶媒 2

HCID 887Methanol溶媒HCID 312751,4-Dioxane溶媒

実験手順

A 25 μl aliquot of the enzyme suspension was added to the above reaction-broth mixture and incubated at 37° C. for 30 minutes. The enzyme reaction was quenched by placing the mixture in a boiling water bath for 10 minutes. A blank was prepared by adding the enzyme aliquot to the reaction mixture after the boiling water treatment. A positive control consisted of a 10 μl mixture of methanol and water (1:1). A 1.0 ml portion of 0.2M K2HPO4 --KH2PO4 buffer (pH=8.3) was added to each boiled mixture and then 0.5 ml of 3% 2,4,6-trichloro-S-triazine in dioxane (3% TT) was added and the mixture vortexed. The solutions were centrifuged and the supernatant from each solution was measured for optical density (O.D.) at 382 nm. The percentage of inhibition of the angiotensin I-converting enzyme was defined by the equation: ##EQU1## Broth samples from medium 3 (see Table I) showing at least 90% inhibition were deemed positive. For the other media listed, a minimum inhibition percentage of 80% indicated a positive result. A positive assay result indicated that the cultured broth was producing the desired compound, 176.

後処理

  1. additionA 25 μl aliquot of the enzyme suspension was added to the above reaction-broth mixture
  2. customThe enzyme reaction
  3. customwas quenched
  4. customfor 10 minutes
  5. customA blank was prepared
  6. customA positive assay result

出典と根拠

ORDレコード
ord-8442d0f681a74d14986aca25c33ec446
出典データセット
uspto-grants-1984_08
特許番号
US04464395

関連ノート

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