Ethylenediaminetetraacetic Acid → Glutathione diethyl ester
反応式
[CH:1]1C(SSC2C=CC([N+]([O-])=O)=C(C(O)=O)C=2)=CC(C(O)=O)=C([N+]([O-])=O)[CH:2]=1.[CH2:27]([C@H:42]([NH2:46])[C:43]([OH:45])=[O:44])[CH2:28][C:29]([NH:31][C@H:32]([C:35]([NH:37][CH2:38][C:39]([OH:41])=[O:40])=[O:36])[CH2:33][SH:34])=[O:30].[CH2:47](N(CC(O)=O)CC(O)=O)[CH2:48]N(CC(O)=O)CC(O)=O>C(O)(=O)C>[CH3:1][CH2:2][O:40][C:39]([CH2:38][NH:37][C:35]([C@@H:32]([NH:31][C:29]([CH2:28][CH2:27][C@H:42]([NH2:46])[C:43]([O:45][CH2:47][CH3:48])=[O:44])=[O:30])[CH2:33][SH:34])=[O:36])=[O:41]参加物
5
実験手順
Analysis of Erythrocyte Thiol by DTNB. Cell supernatant (40 μl) was added to 1 ml of 6 mM DTNB (in 143 mM phosphate buffer, pH 7.5, containing 6.3 mM EDTA). The change in absorbance at 412 nm after 1 min was converted to a value for total thiol using a GSH standard curve. The GSH standard were prepared in a 1:2:2 mixture of PBS: 0.435M acetic acid: 10% SSA (containing 2 mM EDTA) ("RBC Mix").