Formaldehyde → 2-Benzylserine
反応式
P([O-])([O-])([O-])=O.[K+].[K+].[K+].[CH2:9]([C:16]([C:20]([OH:22])=[O:21])([CH2:18][OH:19])[NH2:17])[C:10]1[CH:15]=[CH:14][CH:13]=[CH:12][CH:11]=1.CC1N=CC(COP(O)(O)=O)=C(C=O)C=1O.C=O>>[CH2:9]([C@@:16]([C:20]([OH:22])=[O:21])([CH2:18][OH:19])[NH2:17])[C:10]1[CH:15]=[CH:14][CH:13]=[CH:12][CH:11]=1参加物
5
実験手順
A reaction mixture in a total amount of 0.1 mL obtained by adding 0.03 mL of the cell-free extract solution to a reaction solution A having a composition of 50 mM potassium phosphate buffer (pH 7.4), 10 mM α-benzyl-DL-serine and 0.1 mM pyridoxal phosphate was reacted at 30° C. for 10 minutes. After 10 minutes, the reaction was stopped by mixing 0.1 mL of an alkali reagent (5 N potassium hydroxide) attached in a formaldehyde kit, Test Wako (Wako Pure Chemicals Industries Ltd.).