NADP nicotinamide-adenine-dinucleotide phosphate → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
反応式
P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.C1C=[N+]([C@@H]2O[C@H](COP(OP(OC[C@H]3O[C@@H](N4C5N=CN=C(N)C=5N=C4)[C@H](OP(O)(O)=O)[C@@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=C(C(N)=O)C=1.P([O:84][CH2:85][C@@H:86]([OH:95])[C@@H:87]([OH:94])[C@H:88]([OH:93])[C@@H:89]([OH:92])[CH:90]=[O:91])(O)(O)=O.[Cl-].[Mg+2].[Cl-]>>[O:84]=[CH:85][C@@H:86]([C@H:87]([C@@H:88]([C@@H:89]([CH2:90][OH:91])[OH:92])[OH:93])[OH:94])[OH:95]参加物
5
実験手順
The reagent solution contains albumin, 50 mg/L, adenosine triphosphate 5 mmol/L, NADP (TPN) 500 mg/L, hexokinase 10 units/ml, glucose 6-phosphate dehydrogenase 0.5 units/ml, magnesium chloride 20 mmol/L, all dissolved in 0.1 molar tris buffer pH 8.0.