反応式
[NH:1]1[C:9]2[C:4](=[CH:5][CH:6]=[CH:7][CH:8]=2)[C:3]([CH2:10][C:11](=O)[C:12]([O-:14])=[O:13])=[CH:2]1.[NH2:16][C@H](C(O)=O)CC(O)=O.[Mg+2].[Cl-].[Cl-].[C:28]([CH2:33]C([O-])=O)([C:30]([OH:32])=[O:31])=[O:29]>>[NH2:16][C@H:11]([C:12]([OH:14])=[O:13])[CH2:10][C:3]1[C:4]2[C:9](=[CH:8][CH:7]=[CH:6][CH:5]=2)[NH:1][CH:2]=1.[C:30]([OH:32])(=[O:31])[C:28]([CH3:33])=[O:29]参加物
6
実験手順
A solution of indole-3-pyruvate, 20 mM; L-aspartic acid 20 mM; MgCl2, 1.5 mM; transaminase, 0.3 mg/ml; oxaloacetate decarboxylase, 0.3 mg/ml buffered to pH 7.0 with 5 mM tris-hydroxymethylaminomethane hydrochloride (Tris) was stirred slowly for 2 hours. At the end of this time the reaction is complete. The L-tryptophan and pyruvic acid produced can be purified by methods well known in the art.
出典と根拠
- ORDレコード
- ord-d77010d4b6754d73bea1dc6367e80517
- 出典データセット
- uspto-grants-1985_05
- 特許番号
- US04518692
関連ノート
読み込み中…