Sodium Chloride → Deoxycholic Acid
反応式
[CH3:1][C@@H:2]([C@@H:8]1[C@@:12]2([CH3:29])[C@@H:13]([OH:28])[CH2:14][C@@H:15]3[C@@:20]4([CH3:26])[CH2:21][CH2:22][C@@H:23]([OH:25])[CH2:24][C@H:19]4[CH2:18][CH2:17][C@@:16]3(C)[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([O-:7])=[O:6].[Na+].[Na+].[Cl-]>C(O)C(N)(CO)CO.Cl>[CH3:1][C@@H:2]([C@@H:8]1[C@@:12]2([CH3:29])[C@@H:13]([OH:28])[CH2:14][C@@H:15]3[C@@:20]4([CH3:26])[CH2:21][CH2:22][C@@H:23]([OH:25])[CH2:24][C@H:19]4[CH2:18][CH2:17][C@H:16]3[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([OH:7])=[O:6]参加物
4
実験手順
A pellet containing recombinant prothrombin immobilized on Ca3 (PO4)2 was obtained from a prothrombin-containing cell culture, as described above. Five 0.6 gram samples of moist adsorbate were suspended in 3 ml of 20 mM Tris-HCl buffer (pH 8.3) containing 50 mM, 100 mM, 250 mM, 350 mM or 500 mM sodium deoxycholate. Sixty microliter aliquots of 0.76 mg/ml trypsin were added to each sample, and the mixtures were incubated for three hours at room temperature with shaking. After incubation, the buffers of the samples were changed against 0.9% NaCl.