Diethylenetriaminepentaacetic acid → DTPA dianhydride

HRID 5299

反応式

HRID 5299 の反応式
[CH2:1]([N:12]([CH2:24][C:25]([OH:27])=[O:26])[CH2:13][CH2:14][N:15]([CH2:20][C:21]([OH:23])=O)[CH2:16][C:17]([OH:19])=[O:18])[CH2:2][N:3]([CH2:8][C:9]([OH:11])=[O:10])[CH2:4][C:5]([OH:7])=O>C(Cl)(Cl)Cl>[CH2:20]1[N:15]([CH2:14][CH2:13][N:12]([CH2:24][C:25]([OH:27])=[O:26])[CH2:1][CH2:2][N:3]2[CH2:8][C:9](=[O:10])[O:11][C:5](=[O:7])[CH2:4]2)[CH2:16][C:17](=[O:19])[O:18][C:21]1=[O:23]

参加物

3

反応物 1

HCID 3053Diethylenetriaminepentaacetic acid

生成物 1

HCID 100825DTPA dianhydride

試薬・触媒・溶媒 1

HCID 6212Chloroform溶媒

実験手順

A 0.1 mg/ml solution of DTPA anhydride in dry chloroform was prepared and an aliquot containing the desired quantity of DTPA was added to a reaction vessel. The chloroform was removed by evaporation with nitrogen gas at room temperature. A pH 7.0 solution of antibody (approximately 0.5 mg) in 0.05M bicarbonate buffer was added to said reaction vessel to produce a 7:1 molar ratio of anhydride to protein. The solution was incubated with shaking for one minute and the coupled antibody was recovered by passage over a Sephadex G-50 column.

後処理

  1. additionwas added to a reaction vessel
  2. customThe chloroform was removed by evaporation with nitrogen gas at room temperature
  3. additionA pH 7.0 solution of antibody (approximately 0.5 mg) in 0.05M bicarbonate buffer was added
  4. customreaction vessel
  5. customto produce a 7
  6. customthe coupled antibody was recovered by passage over a Sephadex G-50 column

出典と根拠

ORDレコード
ord-e639ae9580b5409ba9d211431f724411
出典データセット
uspto-grants-1993_09
特許番号
US05244816

関連ノート

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