Potassium Chloride → GlyTouCan:G87605GW

HRID 564889

反応式

HRID 564889 の反応式
[OH:1][C:2]([C:4]1([O:15][C@@H:14]([C@@H:16]([C@@H:18]([CH2:20][OH:21])[OH:19])[OH:17])[C@H:9]([NH:10]C(C)=O)[C@@H:7]([OH:8])[CH2:6]1)[OH:5])=[O:3].[O:22]([C@@H:34]1[C@@H:40]([CH2:41][OH:42])[O:39][CH:37]([OH:38])[C@H:36]([NH:43][C:44]([CH3:46])=[O:45])[C@H:35]1[OH:47])[C@@H:23]1[O:31][C@H:30]([CH2:32]O)[C@H:28]([OH:29])[C@H:26]([OH:27])[C@H:24]1[OH:25].P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.[Cl-].[K+].CC(N[C@H]1C([C@H](O)[C@H](O)CO)O[C@](OP(OC[C@H]2O[C@@H](N3C(=O)N=C(N)C=C3)[C@H](O)[C@@H]2O)([O-])=O)(C(O)=O)C[C@@H]1O)=O.[Na+].C1N(CCO)CCN(CCS(O)(=O)=O)C1.C=CC(N)=O>O>[OH:1][C:2]([C@@:4]1([O:15][C@@H:14]([C@@H:16]([C@@H:18]([CH2:20][OH:21])[OH:19])[OH:17])[C@H:9]([NH2:10])[C@@H:7]([OH:8])[CH2:6]1)[O:5][CH2:32][C@H:30]1[O:31][C@@H:23]([O:22][C@@H:34]2[C@@H:40]([CH2:41][OH:42])[O:39][CH:37]([OH:38])[C@H:36]([NH:43][C:44]([CH3:46])=[O:45])[C@H:35]2[OH:47])[C@H:24]([OH:25])[C@@H:26]([OH:27])[C@H:28]1[OH:29])=[O:3]

参加物

9

反応物 7

HCID 4873Potassium ChlorideHCID 59575'-AtpHCID 6579AcrylamideHCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidHCID 439197N-Acetyl-Neuraminic Acid2 回HCID 9800166beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamineHCID 23679065CMP-NeuAc

生成物 1

HCID 131718308GlyTouCan:G87605GW収率 97%

試薬・触媒・溶媒 1

HCID 962Water溶媒

反応条件

温度
25 °C

実験手順

NeuAc (0.92 g, 3 mmol), Galβ1,4GlcNAc (1.1 g, 3 mmol), CMP (0.1 g, 30 μmol), ATP (16 mg, 3 μmol), PEP (2.8 g, 6 mmol), MgCl2.6H2O (0.61 g, 3 mmol), MnCl2.4H2O (0.15 g, 0.8 mmol), KCl (0.22 g, 3 mmol), NMK or MK (450 U), PK (6,000 U), PPase (300 U), CMP-NeuAc synthetase (24 U), and Siaα2,6Gal sialyl transferase (4 U) were mixed with 150 ml of HEPES buffer (0.2M, pH 7.5) to form a reaction mixture and the reaction mixture maintained under argon at about 25° C. for about 48 hours. After the disappearance of NeuAc (determined by thin-layer chromatography) the reaction mixture was reduced in volume to 20 ml by lyophilization and the lyophilized reaction mixture applied to a Bio Gel P2 (200-400 mesh) column with water as the mobile phase. The trisaccharide-containing fractions were eluted, collected and lyophilized to give pure Neuα2,6Galβ1,4GlcNAc in 97 percent yield.

後処理

  1. customto form a reaction mixture
  2. washwere eluted
  3. customcollected

出典と根拠

ORDレコード
ord-8a26a2b1eaf64300969b48ff4d15de50
出典データセット
uspto-grants-2001_01
特許番号
US06168934A

関連ノート

読み込み中…