反応式
[CH2:1]1[S:5][C@@H:4]([CH2:6][CH2:7][CH2:8][CH2:9][C:10](NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=[O:11])[C@H:3]2[NH:32][C:33]([NH:35][C@@H:2]12)=[O:34].[Na+].[OH:37]P([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+].OP([O-])(O)=O.[K+].OP([O-])([O-])=O.[K+].[K+].[Na+].[Cl-].Cl.CN1C(=O)C=CS1.[Cl-].[Cl-].[Ca+2]>>[OH:37][C:10]([CH2:9][CH2:8][CH2:7][CH2:6][C@H:4]1[C@@H:3]2[C@@H:2]([NH:35][C:33]([NH:32]2)=[O:34])[CH2:1][S:5]1)=[O:11]参加物
反応物 7
生成物 1
実験手順
An EZ-Link® Sulfo-NHS-LC Biotin stock (Cat: 23224, Thermo Fisher Scientific, Rockford, Ill.) was freshly prepared by adding 300 μL of ice-cold water in a single polypropylene microcentrifuge tube containing 1.0 mg of EZ-LINK® Sulfo-NHS-LC Biotin resulting in a concentration of 5.99 nMole/μL. To a 0.25 mL solution of ANTI-FLAG® M1 antibody (4.041 mg/mL) in a microcentrifuge tube, 11 μL of the freshly prepared EZ-LINK® Sulfo-NHS-LC Biotin water solution was added. This solution was incubated and mixed continuously for 1 hour at room temperature. After the incubation, the antibody was buffer exchanged to a phosphate buffered saline (PBS) containing 37.5 mM KH2PO4, 112.5 mM K2HPO4, 150.6 mM NaCl, 0.10% 2-Methyl-4-isothiazolin-3-one Hydrochloride (MIT), and 7 mM CaCl2. The post modification concentration was 0.975 mg/mL in approximately 1.0 mL.
後処理
- additionmixed continuously for 1 hour at room temperature
出典と根拠
- ORDレコード
- ord-252dadc10cf547ed8996e22126629277
- 出典データセット
- uspto-grants-2016_01
- 特許番号
- US09244073B2
関連ノート
読み込み中…