Methylene blue cation → 1H-Indolamine

HRID 69826

反応式

HRID 69826 の反応式
[CH:1]1[CH:2]=[CH:3][C:4]2[NH:9][CH:8]=[C:7](C[C@@H](N)C(O)=O)[C:5]=2[CH:6]=1.O=C1O[C@H]([C@H](CO)O)C([O-])=C1O.C[N:29](C1C=CC2N=C3C(=CC(C=C3)=[N+](C)C)SC=2C=1)C>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[NH:9]1[C:4]2[C:5](=[CH:6][CH:1]=[CH:2][CH:3]=2)[CH:7]=[C:8]1[NH2:29]

参加物

5

反応物 3

HCID 4139Methylene blue cationHCID 9060D-TryptophanHCID 178519208HCID 178519208、化合物

生成物 1

HCID 1220741H-Indolamine

試薬・触媒・溶媒 1

HCID 62657Potassium Phosphate, Tribasic溶媒

実験手順

Human indoleamine 2,3-dioxygenasae (IDO) with an N-terminal His tag was expressed in E. coli and purified to homogeneity. IDO catalyzes the oxidative cleavage of the pyrrole ring of the indole nucleus of tryptophan to yield N′-formylkynurenine. The assays were performed at room temperature as described in the literature using 95 nM IDO and 2 mM D-Trp in the presence of 20 mM ascorbate, 5 μM methylene blue and 0.2 mg/mL catalase in 50 mM potassium phosphate buffer (pH 6.5). The initial reaction rates were recorded by continuously following the absorbance increase at 321 nm due to the formation of N′-formlylkynurenine (See: Sono, M., et al., 1980, J. Biol. Chem. 255, 1339-1345).

後処理

  1. customwere performed at room temperature
  2. customThe initial reaction rates
  3. temperatureincrease at 321 nm due to the formation of N′-formlylkynurenine (See: Sono, M., et al., 1980, J. Biol. Chem. 255, 1339-1345)

出典と根拠

ORDレコード
ord-e9c2aa91fd504d05a76864c21cf0a5fa
出典データセット
uspto-grants-2014_09
特許番号
US08822511B2

関連ノート

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