Chloramphenicol → isopropyl beta-D-thiogalactopyranoside

HRID 713537

反応式

HRID 713537 の反応式
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[NH4+].[Cl-].[CH3:15][C:16]1(C)[S:20][C@@H]2[C@H](NC(C(C(O)=O)C3C=CC=CC=3)=O)C(=O)N2[C@H:17]1C(O)=O.C1C([C@@H](O)[C@H](NC(C(Cl)Cl)=O)CO)=CC=C([N+]([O-])=O)C=1.C1[C@H](N)[C@@H](O[C@H]2O[C@H](CN)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](N)[C@H]2O)[C@@H]1N>C1N(CCCS(O)(=O)=O)CCOC1>[CH3:15][CH:16]([S:20][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4])[CH3:17]

参加物

7

反応物 5

HCID 5959Chloramphenicol2 回HCID 6032KanamycinHCID 20824CarbenicillinHCID 25517Ammonium ChlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

生成物 1

HCID 656894isopropyl beta-D-thiogalactopyranoside

試薬・触媒・溶媒 1

HCID 70807Mops溶媒

実験手順

All liquid cultivations were conducted in MOPS minimal medium (Teknova) [14] cultures supplemented with 5 g/l glucose and an additional 4 g/l NH4Cl. When appropriate, antibiotics were added in the following concentrations: 100 μg/ml carbenicillin for the maintenance of pTrc- or pET-derived plasmids, 34 μg/ml chloramphenicol for pHACM-derived plasmids, 68 μg/ml chloramphenicol for pACYC-derived plasmids and pRARE2 (Novagen), and 20 μg/ml kanamycin for pACKm-derived plasmids. Isopropyl-β-D-thiogalactopyranoside (IPTG, EMD Chemicals) was provided at a concentration of 1 mM for the induction of expression from both trc and T7 promoters. Cultures for L-phenylalanine auxotrophic (ΔpheA) strains were additionally supplemented with L-phenylalanine (Sigma) at a concentration of 0.35 mM. For malonyl CoA availability experiments, cerulenin (Cayman Chemicals) and sodium malonate dibasic (Sigma) were added at a concentration 20 μg/ml and 2 g/l (1 g/l added twice), respectively.

後処理

  1. additionWhen appropriate, antibiotics were added in the following concentrations

出典と根拠

ORDレコード
ord-25ca403495bc4782b318fa2b4273bf7b
出典データセット
uspto-grants-2015_11
特許番号
US09181539B2

関連ノート

読み込み中…