Platinum → HCID 178443123、化合物

HRID 841004

反応式

HRID 841004 の反応式
[Pt:1].[C:2]([O:19]C(=O)CCCCCCCCCCCCCCC)(=O)[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]>C(=O)(O)[O-].C([NH+](CC)CC)C.C(O)(C)C.O.[Pd]>[C:2]([Pt:1])(=[O:19])[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]

参加物

6

反応物 2

HCID 23939PlatinumHCID 69339Palmitic anhydride

生成物 1

HCID 178443123HCID 178443123、化合物

試薬・触媒・溶媒 3

HCID 23938Palladium触媒HCID 11768884Triethylammonium hydrogen carbonate溶媒HCID 21889133Propan-2-ol--water (1/1)溶媒

実験手順

Preparation used the same procedure as the platinum cluster conjugate: an estimated 30 nmol of amino-derivatized 2-3 nm palladium cluster, dissolved in 0.6 M triethylammonium bicarbonate in 20% isopropanol/water, was evaporated to dryness five times from methanol, dissolved in 3 mL of dichloromethane, then treated with 200 nmol of palmitic anhydride. The mixture was stirred for one hour at room temperature, washed three times with 0.1 M sodium phosphate buffer at pH 6.5, evaporated to dryness, redissolved in 2:1 methanol/chloroform and separated by gel filtration (GH25, Amicon, eluted with 2:1 methanol:chloroform). After evaporation to dryness, lipid conjugation was demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate was shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent.

後処理

  1. customPreparation
  2. customwas evaporated to dryness five times from methanol
  3. dissolutiondissolved in 3 mL of dichloromethane
  4. washwashed three times with 0.1 M sodium phosphate buffer at pH 6.5
  5. customevaporated to dryness
  6. dissolutionredissolved in 2:1 methanol/chloroform
  7. customseparated by gel filtration (GH25, Amicon
  8. washeluted with 2:1 methanol
  9. customAfter evaporation to dryness, lipid conjugation
  10. extractionwas demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate
  11. stirringwas shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent

出典と根拠

ORDレコード
ord-82b5e0c06bf94248b0ea42ee77367dc3
出典データセット
uspto-grants-2002_04
特許番号
US06369206B1

関連ノート

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