反応式
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4]P(O)(O)=O)(=[O:3])[OH:2].P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+].C(O)C(N)(CO)CO.Cl.[Mg]>>[CH:23]1[N:22]=[C:20]([NH2:21])[C:19]2[N:18]=[CH:17][N:16]([C@@H:14]3[O:15][C@H:11]([CH2:10][O:9][P:1]([OH:4])([OH:3])=[O:2])[C@@H:12]([OH:27])[C@H:13]3[OH:26])[C:25]=2[N:24]=1参加物
6
実験手順
A second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow is included for feeding very pure adenosine diphosphate (ADP) reagent, with respect to ATP, into the processing fluid downstream of the junction (5) where detergent is added, but upstream of the luminometer. The ADP reagent (99.95% pure with respect to ATP) is added at a flow and concentration such as to produce a concentration of 0.5 mM in the final flow. The processing fluid is pH 7.4 phosphate buffered saline or TrisHCl pH 7.8 including 0.2 mM magnesium ions.
後処理
- customA second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow
- customto produce a concentration of 0.5 mM in the final flow
出典と根拠
- ORDレコード
- ord-f46e2aa47c954c5db35f79c7548b9286
- 出典データセット
- uspto-grants-1998_06
- 特許番号
- US05773710
関連ノート
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