Dimethyl Sulfoxide → 1-Methyl-2,4,6(1H,3H,5H)-pyrimidinetrione

HRID 1181672

반응식

HRID 1181672 반응식
C(OC(=O)C)(=O)C.[C:8]([OH:14])(=O)[CH2:9][C:10]([OH:12])=O.[CH3:15][NH:16][C:17]([NH2:19])=[O:18].CS(C)=O>C(O)(=O)C>[CH3:15][N:16]1[C:8](=[O:14])[CH2:9][C:10](=[O:12])[NH:19][C:17]1=[O:18]

참가 물질

6

반응물 4

HCID 679Dimethyl SulfoxideHCID 867Malonic AcidHCID 7918Acetic AnhydrideHCID 11719Methylurea

생성물 1

HCID 757221-Methyl-2,4,6(1H,3H,5H)-pyrimidinetrione

시약·촉매·용매 1

HCID 176Acetic Acid용매

반응 조건

온도
95 °C

실험 절차

Acetic anhydride (34 ml) was added to a solution of malonic acid (20 g) and methylurea (12.5 g) in acetic acid (46 ml). The mixture was heated to 95° C. for 3 hours then cooled to room temperature. The resulting solution was concentrated under reduced pressure and the residue as 20 mM stock solutions in dimethyl sulphoxide (DMSO, Sigma #D-5879) and serial dilutions were prepared from these solutions using DMSO, 2 μL of which were added to the wells. Activity at room temperature was measured by monitoring the increase in fluorescence using a TECAN Ultra platereader with 340 nm excitation and 465 nm emission filters. The compounds provided have measured IC50 of less then 400 μM

후처리

  1. temperaturethen cooled to room temperature
  2. concentrationThe resulting solution was concentrated under reduced pressure
  3. customthe residue as 20 mM stock solutions in dimethyl sulphoxide (DMSO, Sigma #D-5879) and serial dilutions were prepared from these solutions
  4. additionwere added to the wells
  5. customat room temperature
  6. temperatureincrease in fluorescence
  7. customThe compounds provided

출처 및 근거

ORD 기록
ord-13c17c6528dd44a7adb4f6d33a066515
출처 데이터셋
uspto-grants-2007_10
특허 번호
US07285558B2

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