Lithium Hydroxide → (2S)-2-Boc-aminomethyl-3-(4-chlorophenyl)-1-[4-(5-thiophen-2-yl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)-pip-erazin-1-yl]-propan-1-one

To a solution of 7-Benzenesulfonyl-4-piperazin-1-yl-5-thiophen-2-yl-7H-pyrrolo[2,3-d]pyrimidine dihydrochloride (50 mg, 0.10 mmol), DIEA (0.10 mL, 0.60 mmol), and (2S)-2-(Boc-aminomethyl)-3-(4-chlorophenyl)-propionic acid (38 mg, 0.12 mmol) in 2 mL DCM was added HBTU (44 mg, 0.12 mmol). The reaction mixture was stirred at room temperature 2 hours, after which 2 mL MeOH and 0.5 mL 3M LiOH were added. The reaction mixture was heated to 35° C. and stirred 2 hours, after which saturated NaHCO3 was added, and the mixture was extracted with DCM. The combined extracts were dried (Na2SO4), filtered, and concentrated. The crude was purified on silica gel (flushed with 2:1 DCM:EtOAc, then gradient to 1:4 DCM:EtOAc) to give (2S)-2-Boc-aminomethyl-3-(4-chlorophenyl)-1-[4-(5-thiophen-2-yl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)-pip-erazin-1-yl]-propan-1-one, which was used in the next step.

1. temperatureThe reaction mixture was heated to 35° C.
2. stirringstirred 2 hours
3. extractionthe mixture was extracted with DCM
4. dry with materialThe combined extracts were dried (Na2SO4)
5. filtrationfiltered
6. concentrationconcentrated
7. customThe crude was purified on silica gel (
8. customflushed with 2:1 DCM

ORD 기록

ord-3ba1dc7d18354748928301605e50ad77

출처 데이터셋

uspto-grants-2014_03

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US08680114B2