Hydrochloric Acid → Carbobenzoxy-tyrosyl-glycyl-glycine
반응식
[NH:1]([C:15]([O:17][CH2:18][C:19]1[CH:24]=[CH:23][CH:22]=[CH:21][CH:20]=1)=[O:16])[C@H:2]([C:11]([O:13]C)=O)[CH2:3][C:4]1[CH:9]=[CH:8][C:7]([OH:10])=[CH:6][CH:5]=1.[NH2:25][CH2:26][C:27]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:28].Cl>CS(C)=O>[NH:1]([C:15]([O:17][CH2:18][C:19]1[CH:24]=[CH:23][CH:22]=[CH:21][CH:20]=1)=[O:16])[C@H:2]([C:11]([NH:25][CH2:26][C:27]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:28])=[O:13])[CH2:3][C:4]1[CH:5]=[CH:6][C:7]([OH:10])=[CH:8][CH:9]=1참가 물질
5
실험 절차
330 mg (1 mmole) of Z-L-Tyr-OMe, 400 mg (2.2 mmole) of H-Gly-Gly-Ome, HCl were suspended in 5 mL DMSO and 5 mL 0.1 M tris-HCl, pH 8.9. 25 mg of α-chymotrypsin were added and the reaction stirred for 30 minutes. Five volumes of ice water were added, and the precipitate collected by filtration. Yield 312 mg (70%, based on acyl-donor). Amino acid analysis: tyr1.0,gly2.1.