(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → Ribosylthymidine diphosphate

HRID 1280309

반응식

HRID 1280309 반응식
[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([CH3:28])[C:24](=[O:25])[NH:23][C:21]2=[O:22])[C@H:17]([OH:29])[C@@H:16]1[OH:30])([O:4][P:5]([O:8]P(O)(O)=O)([OH:7])=[O:6])(=[O:3])[OH:2].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[O-]P(OP([O-])([O-])=O)(=O)[O-].[O-]P(OP(OP([O-])([O-])=O)([O-])=O)(=O)[O-]>C(O)C(N)(CO)CO.S([O-])([O-])(=O)=O.[NH4+].[NH4+]>[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([CH3:28])[C:24](=[O:25])[NH:23][C:21]2=[O:22])[C@H:17]([OH:29])[C@@H:16]1[OH:30])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:2])[OH:3]

참가 물질

7

반응물 4

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 4513885-Methyluridine 5'-triphosphateHCID 644102Pyrophosphate2회HCID 3440921Triphosphate2회

생성물 1

HCID 23379797Ribosylthymidine diphosphate

시약·촉매·용매 2

HCID 6503Tris(hydroxymethyl)aminomethane용매2회HCID 6097028Ammonium Sulfate용매

실험 절차

5-Methyluridine 5′-triphosphate (0.0047 g, 0.009 mMol) was dissolved in 0.7 mL 1M Tris buffer (pH 8.5 containing 1M MgCl2) then 0.3 mL of a 0.25M glucose solution in 1M Tris buffer (pH 8.5 containing 1M MgCl2) added. A To timepoint was taken by reverse phase anion exchange HPLC. Hexokinase (EC 2.7.1.1, Boehringer Mannheim, from yeast overproducer), 75 units in 3.2M ammonium sulfate suspension, was added to the reaction which was incubated at 25° C. An HPLC was taken at 66 hours indicating complete conversion to diphosphate with no triphosphate remaining. Triphosphate retention: 17.5 minutes; diphosphate retention: 10.3 minutes.

후처리

  1. customwas incubated at 25° C
  2. custom17.5 minutes
  3. custom10.3 minutes

출처 및 근거

ORD 기록
ord-f7b2247915384ecb88c46a7bdbef90cc
출처 데이터셋
uspto-grants-2002_09
특허 번호
US06451288B1

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