L-Valine → L-Alanine

HRID 1382180노트

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HRID 1382180 반응식
[NH2:1][C@H:2]([C:6]([OH:8])=[O:7])[CH:3](C)C.N(C(C1C=CC=CC=1)=O)[C@H](C(OC)=O)C.[OH-].[Na+].N(C(C1C=CC=CC=1)=O)[C@H](C([NH:31][C@H:32]([C:36]([OH:38])=[O:37])[CH:33]([CH3:35])[CH3:34])=O)C>Cl>[NH2:1][C@H:2]([C:6]([OH:8])=[O:7])[CH3:3].[NH2:31][C@H:32]([C:36]([OH:38])=[O:37])[CH:33]([CH3:35])[CH3:34]

참가 물질

7

반응물 4

HCID 6287L-ValineHCID 14798Sodium HydroxideHCID 63646177244-67-9HCID 54510848HCID 54510848, 화합물

생성물 2

HCID 5950L-AlanineHCID 6287L-Valine

시약·촉매·용매 1

HCID 313Hydrochloric Acid용매2회

실험 절차

A solution of 2 ml of 0.6M Valine-0.1M KCl-1 mM EDTA, pH 9.8 was mixed with 100 μl (0.1 mmole) of a 1M Bz-Ala-OMe (dissolved in 96% ethanol) solution. The reaction was carried out in a pH-stat at 35° C. and pH 9.8, the pH-value being kept constant by automatic addition of 0.5M NaOH. The reaction was initiated by adding 0.7 mg of carboxypeptidase Y (150 U/mg, prepared by De Forenede Bryggerier). After a reaction time of 30 minutes, the reaction was discontinued by adjusting pH to about pH=1 with 6M HCl. The reaction product was purified and isolated by means of high pressure chromatography. The yield of Bz-Ala-Val-OH was 40%. Quantitative amino acid analysis after hydrolysis of the product in 6M HCl for 24 hours gave relatively 1.0 mole of Alanine and 1.0 mole of Valine.

후처리

  1. customwas carried out in a pH-stat at 35° C.
  2. additionby adding
  3. custom0.7 mg of carboxypeptidase Y (150 U/mg, prepared by De Forenede Bryggerier)
  4. customAfter a reaction time of 30 minutes
  5. customThe reaction product was purified
  6. customisolated by means of high pressure chromatography

출처 및 근거

ORD 기록
ord-42a9b3357a2b4e55a852306386a0160b
출처 데이터셋
uspto-grants-1989_02
특허 번호
US04806473

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