C[O:2][C:3](=[O:23])[C:4]1[CH:9]=[C:8]([N:10]([S:12]([CH:15]2[CH2:17][CH2:16]2)(=[O:14])=[O:13])[CH3:11])[N:7]=[C:6]([NH:18][C@H:19]([CH2:21][CH3:22])[CH3:20])[CH:5]=1.[OH-].[Na+].Cl>CO>[C@@H:19]([NH:18][C:6]1[CH:5]=[C:4]([CH:9]=[C:8]([N:10]([S:12]([CH:15]2[CH2:16][CH2:17]2)(=[O:13])=[O:14])[CH3:11])[N:7]=1)[C:3]([OH:23])=[O:2])([CH2:21][CH3:22])[CH3:20]Dissolve (S)-2-sec-butylamino-6-(cyclopropanesulfonyl-methylamino)-isonicotinic acid methyl ester (600 mg, 1.97 mmol) in methanol (18 mL). Slowly add 2 N NaOH (3 mL) and stir overnight at room temperature. Acidify the mixture to about pH=6 with 5 N HCl and concentrate. Dilute with ethyl acetate (30 mL) and wash the organic layer with saturated aqueous sodium chloride solution, dry (magnesium sulfate) and concentrate to give the title compound.
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