2-Mercaptoethanol → 4-Methylumbelliferone

HRID 1436968

반응식

HRID 1436968 반응식
SCCO.C(N(CC([O-])=O)CC(O)=O)CN(CC([O-])=O)CC(O)=O.[Na+].[Na+].CCCCCCCCCCCCOS([O-])(=O)=O.[Na+].CCC(COC(C(N(CC[NH+](C)C)C)=O)(C1C=CC=CC=1)C1C=CC=CC=1)CC.[Cl-].[CH3:75][C:76]1[C:82]2[CH:83]=[CH:84][C:85]([O:87][C@@H]3O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]3O)=[CH:86][C:81]=2[O:80][C:78](=[O:79])[CH:77]=1>C([O-])([O-])=O.[Na+].[Na+]>[CH3:75][C:76]1[C:82]2[CH:83]=[CH:84][C:85]([OH:87])=[CH:86][C:81]=2[O:80][C:78](=[O:79])[CH:77]=1

참가 물질

7

반응물 5

HCID 15672-MercaptoethanolHCID 8759EDTA disodium saltHCID 19153X 100HCID 915534-Methylumbelliferyl beta-D-glucuronideHCID 3423265Sodium Lauryl Sulfate

생성물 1

HCID 52805674-Methylumbelliferone

시약·촉매·용매 1

HCID 10340Sodium Carbonate용매

실험 절차

To evaluate the activity of various promoters, GUS fluorescence was assayed by the method of Jefferson (1987). Maturing seeds at 17 DAF were homogenized in GUS extraction buffer (50 mM NaPO4 [pH 7.0], 10 mM 2-mercaptoethanol, 10 mM Na2-EDTA, 0.1% SDS, 0.1% Triton X-100). After centrifugation, 10 μl of the supernatant was mixed with 90 μl of assay buffer containing 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG). After incubating for one hour at 37° C., 900 μl of 0.2 M Na2CO3 was added to the mixture to terminate the reaction. Values obtained using a fluorometer were compared with those obtained from serial dilutions of 4-methylumbelliferone (4MU). The protein amount was determined using a BIO-RAD® Protein Assay kit, which is based on the Bradford method (Bradford, M., Anal. Biochem. 72, 248 (1976)), with serum albumin as the standard. Three seeds were assayed for each transgenic plant.

후처리

  1. additionAfter centrifugation, 10 μl of the supernatant was mixed with 90 μl of assay
  2. customto terminate
  3. customthe reaction
  4. customValues obtained

출처 및 근거

ORD 기록
ord-fe140407f7ab4885918f418d47bdaca3
출처 데이터셋
uspto-grants-2009_11
특허 번호
US07619135B2

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