L-Glutamine → HCID 72193709, 화합물

HRID 1591714

반응식

HRID 1591714 반응식
O=C[C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].N[C@H](C(O)=O)C[CH2:16][C:17](=[O:19])N.CC1N=CC(CO)=C(CO)C=1O.Cl.C([O-])(=O)C(C)=O.[Na+].CC1(C)S[C@@H]2[C@H](NC(CSCC=C)=O)C(=O)N2[C@H]1C(O)=O.C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.CC(OC1O[C@H](C[O:114][P:115]([O:118][P:119]([O:122][CH2:123][C@H:124]2[O:128][C@@H:127]([N:129]3[C:133]4[N:134]=[CH:135][N:136]=[C:137]([NH2:138])[C:132]=4[N:131]=[CH:130]3)[C@H:126]([OH:139])[C@@H:125]2[OH:140])([OH:121])=[O:120])(O)=[O:116])[C@@H](O)[C@H]1O)=O>>[CH3:16][C:17]([O:10][C@H:9]1[CH:11]([OH:12])[O:6][C@H:5]([CH2:3][O:4][P:115]([O:118][P:119]([O:122][CH2:123][C@H:124]2[O:128][C@@H:127]([N:129]3[C:133]4[N:134]=[CH:135][N:136]=[C:137]([NH2:138])[C:132]=4[N:131]=[CH:130]3)[C@H:126]([OH:139])[C@@H:125]2[OH:140])([OH:121])=[O:120])([OH:116])=[O:114])[C@H:7]1[OH:8])=[O:19]

참가 물질

8

반응물 7

HCID 5961L-GlutamineHCID 6019Pyridoxine HydrochlorideHCID 71365AlmecillinHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 23662274Sodium pyruvateHCID 1011006211-O-acetyl-ADP riboseHCID 178484872HCID 178484872, 화합물

생성물 1

HCID 72193709HCID 72193709, 화합물

실험 절차

HeLa Cells were grown at 37° C. in Dulbecco's Modified Eagles Medium containing D-glucose 1 g/L, L-glutamine 1 g/L, pyridoxine hydrochloride 1 g/L, sodium pyruvate 110 mg/L, 10% fetal bovine serum (FBS), penicillin at 1000 units/mL, and streptomycin at 1 mg/mL (Gibco BRL) (growth medium). When cells had reached 80-90% confluency, they were rinsed once with PBS, lysed in 100-200 μL of ice-cold, 20 mM Tris, pH 7.2, 137 mM NaCl, 10% glycerol, 1% Nonidet P-40, 100 μM phenylmethylsulfonyl fluoride (PMSF), 20 μg/mL leupeptin, and 20 μg/mL of aprotinin. Cells were scraped into eppendorf tubes, sonicated for 10s, and centrifuged at 16,000×g for 10 min to remove cell debris. Protein concentrations of supernatants were determined by the method of Bradford. Lysates (40 μL) of the cells or buffer controls were then incubated with radiolabeled 1-O-acetyl-ADP ribose (about 0.5 nmol) (total volume of reaction was 100 μL) for the indicated times prior to analysis by reverse phase HPLC. The HPLC analysis was carried out as described previously.

후처리

  1. customwere grown at 37° C. in Dulbecco's Modified Eagles Medium
  2. washwere rinsed once with PBS
  3. customsonicated for 10s
  4. customto remove cell debris

출처 및 근거

ORD 기록
ord-b59186c1b94945969467a589c6844f24
출처 데이터셋
uspto-grants-2007_11
특허 번호
US07291606B2

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