Kanamycin A Sulfate → Kanamycin A Sulfate

HRID 1606394

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HRID 1606394 반응식
[CH2:1]1[C@H:6]([NH2:7])[C@@H:5]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][NH2:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:4]([OH:20])[C@@H:3]([O:21][C@H:22]2[O:27][C@H:26]([CH2:28][OH:29])[C@@H:25]([OH:30])[C@H:24]([NH2:31])[C@H:23]2[OH:32])[C@@H:2]1[NH2:33].[OH:34][S:35]([OH:38])(=[O:37])=[O:36]>O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>[CH2:1]1[C@H:6]([NH2:7])[C@@H:5]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][NH2:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:4]([OH:20])[C@@H:3]([O:21][C@H:22]2[O:27][C@H:26]([CH2:28][OH:29])[C@@H:25]([OH:30])[C@H:24]([NH2:31])[C@H:23]2[OH:32])[C@@H:2]1[NH2:33].[CH2:1]1[C@H:6]([NH2:7])[C@@H:5]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][NH2:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:4]([OH:20])[C@@H:3]([O:21][C@H:22]2[O:27][C@H:26]([CH2:28][OH:29])[C@@H:25]([OH:30])[C@H:24]([NH2:31])[C@H:23]2[OH:32])[C@@H:2]1[NH2:33].[OH:37][S:35]([OH:38])(=[O:36])=[O:34]

참가 물질

4

반응물 1

HCID 441374Kanamycin A Sulfate

생성물 2

HCID 6032KanamycinHCID 441374Kanamycin A Sulfate수율 186%

시약·촉매·용매 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal용매

반응 조건

온도
120 °C

실험 절차

Simulated kanamycin culture broth is prepared by adding 80 mg of kanamycin sulfate to 160 ml of a glucose bouillon medium, which s sterilized under heating at 120°C for 15 minutes and cooled. The culture broth is then passed through a column of 1.5 cm in diameter packed with 30 g of the selective adsorbing particles obtained in Example 2 at a flowing velocity of 0.5 ml/minute. The column is then washed with water and further eluted with 0.01 N HCl-methanol (1 : 1). Respective active fractions are combined to give the result that 74.4 mg (recovery percentage: 91%) of kanamycin sulfate is obtained in very high purity and yield with 98% decoloration degree and 98% purity.

후처리

  1. temperaturecooled
  2. customobtained in Example 2 at a flowing velocity of 0.5 ml/minute
  3. washThe column is then washed with water
  4. washfurther eluted with 0.01 N HCl-methanol (1 : 1)
  5. customto give the

출처 및 근거

ORD 기록
ord-2dd6c1c2dae847c0929248c9cc62f32d
출처 데이터셋
uspto-grants-1976_04
특허 번호
US03953360

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