Erythrose, 4-phosphate → 3-Dehydroshikimate

HRID 1710321노트

반응식

HRID 1710321 반응식
P(O[CH2:6][C@@H:7]([OH:12])[C@@H:8]([OH:11])[CH:9]=[O:10])(O)(O)=O.[C:13]([O-:18])(=[O:17])[C:14]([CH3:16])=O.[Na+].C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1>>[CH2:16]1[C:14]([C:13]([OH:18])=[O:17])=[CH:6][C:7](=[O:12])[C@@H:8]([OH:11])[C@@H:9]1[OH:10]

참가 물질

4

반응물 3

HCID 122357Erythrose, 4-phosphateHCID 439153NadhHCID 23662274Sodium pyruvate

생성물 1

HCID 4397743-Dehydroshikimate수율 90%

실험 절차

D-Erythrose 4-phosphate (0.45 mL, 12 mM, pH 7.0), sodium pyruvate (0.054 mL, 100 mM, pH 7.0), CoCl2 (0.027 mL, 10 mM), NAD (0.054 mL, 1 mM), DHQ synthase (2 units) and DHQ dehydratase (2 units) are incubated with KDPGal aldolase (100 units) from E. coli at room temperature for 2 h. Protein is subsequently removed by ultrafiltration using Millipore PM-10 membrane. 3-Dehydroshikimate was formed in 90% yield, as determined by 1H NMR analysis.

후처리

  1. customProtein is subsequently removed by ultrafiltration

출처 및 근거

ORD 기록
ord-1f061e6c70e247289b3a99639d848c94
출처 데이터셋
uspto-grants-2010_09
특허 번호
US07790431B2

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