Bicine → Acetate

반응식

[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Li+].[O:8]=[C:9]([OH:18])[CH2:10]N(CCO)CCO.C([O-])(=O)CO.[OH-].[Na+]>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:9]([O-:18])(=[O:8])[CH3:10]

참가 물질

반응물 4

HCID 8761Bicine

HCID 14798Sodium Hydroxide

HCID 5460308Hydroxyacetate

HCID 23687877Lithium (S)-2-hydroxypropanoate

생성물 2

HCID 175Acetate

HCID 107735Pyruvate

실험 절차

The procedure described in Comparative Example A was repeated using a 10 mL aqueous solution containing lithium L-lactate (0.750M), FMN (0.01 mM), isobutyfic acid (HPLC internal standard, 0.100M), bicine buffer (0.788M), soluble spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 8.9 (adjusted with 50% NaOH) and the reaction run at 5° C. After 28.5 hours, the HPLC yields of pyruvate and acetate were 47.7% and 43.6%, respectively, and 11.5% lactate remained. The remaining activity of glycolate oxidase and catalase were 40% and 100%, respectively, of their initial values.